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当前位置: 首页 > 文章 > 菊叶香藜精油合成关键HMGR基因的生物信息学分析 河南农业科学 2018 (7) 59-66+89
Position: Home > Articles > Bioinformatics Analysis of Pivotal HMGR Gene Involved in Essential Oil Biosynthesis of Dysphania schraderiana Journal of Henan Agricultural Sciences 2018 (7) 59-66+89

菊叶香藜精油合成关键HMGR基因的生物信息学分析

作  者：
付苏宏;雷鸣;张勇群;施静;郝豆豆
关键词：
菊叶香藜;萜类化合物;3-羟基-3-甲基戊二酰辅酶A还原酶(HMGR);生物信息学
摘  要：
采用生物信息学的方法分析菊叶香藜3-羟基-3-甲基戊二酰辅酶A还原酶(Ds HMGR)基因所编码蛋白质的理化性质、信号肽、跨膜结构域、亚细胞定位、保守结构域、二级结构、三级结构,并进行多条氨基酸序列比对和系统进化树构建。结果表明,Ds HMGR基因包含一段1 725 bp的开放阅读框,编码574个氨基酸,非极性氨基酸为主要氨基酸,等电点为7.46,为不稳定蛋白质,编码蛋白质中不含有信号肽,在N端具有2个疏水区域,含有2个跨膜结构域,Ds HMGR蛋白定位到内质网上,在C端和连接区具有较高保守性,在N端保守性较低,含有HMGR蛋白特有的4个保守活性位点,二级结构主要为α-螺旋,三级结构预测显示Ds HMGR蛋白具有N、L和S 3个催化活性中心结构域。HMGR蛋白进化分析中,菊叶香藜与藜科植物聚为一支,遗传距离较近。
译  名：
Bioinformatics Analysis of Pivotal HMGR Gene Involved in Essential Oil Biosynthesis of Dysphania schraderiana
作  者：
FU Suhong;LEI Ming;ZHANG Yongqun;SHI Jing;HAO Doudou;Molecular Medical Laboratory,Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region;
单  位：
FU Suhong%LEI Ming%ZHANG Yongqun%SHI Jing%HAO Doudou%Molecular Medical Laboratory,Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region
关键词：
Dysphania schraderiana;;Terpenoid;;3-hydroxy-3-methyl glutaryl coenzyme A reductase(HMGR);;Bioinformatics
摘  要：
The physical and chemical properties,secondary structures,signal peptide,transmembrane domain,subcellular localization,conserved domain,tertiary structure and evolutionary relationship of Ds HMGR protein were predicted and analyzed by a series of bioinformatics software. As results,Ds HMGR gene contained an open reading box of 1 725 bp. Ds HMGR was composed of 574 amino acids,among which most were non-polar amino acids. Ds HMGR was an unstable protein with the p I of 7. 46. In addition,Ds HMGR possessed two transmembrance domains anchoring the deduced protein to endoplasmic reticulum and two hydrophobic areas,but no signal peptide was found. The main secondary structure of Ds HMGR was α-helix spread throughout the peptide chain. The conservation in the C-terminus region with four conserved active sites and high variation in the N-terminus region compared with other plants were observed and might provide similar catalytic activity and disparate regulatory process of HMGR activity. Tertiary structure prediction resulted in the observation of three catalytic domains. Ds HMGR was clustered into Chenopodiaceae plants,indicating the closest phylogenetic relationship.

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