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Position: Home > Articles > Cloning and Sequence Analysis of AFP Gene Conservative Region in Ammopiptanthus Nanus Xinjiang Agricultural Sciences 2009,46 (6) 1307-1310

新疆沙冬青抗冻蛋白基因保守区序列的克隆及其分析

作  者:
詹彬;安沙舟;杨丽娟;李莲瑞;马春晖
单  位:
新疆农业大学草业与环境科学学院;塔里木大学动物科学学院/新疆兵团塔里木畜牧科技重点实验室
关键词:
新疆沙冬青;抗冻蛋白;克隆;序列分析
摘  要:
【目的】从Genbank数据库中选择与新疆沙冬青(Ammopiptanthus nanus)亲缘关系最近的同属蒙古沙冬青(Ammopiptanthus mongolicus)抗冻蛋白(AFP)基因AY590122、AY843521、AY843522的mRNA序列,设计扩增新疆沙冬青AFP基因保守区引物。【方法】以新疆沙冬青为研究材料,提取其总RNA,利用RT-PCR技术克隆出新疆沙冬青AFP基因的保守区域。【结果】序列分析表明,新疆沙冬青与蒙古沙冬青的保守区域同源性达98.15%。【结论】得到新疆沙冬青的抗冻蛋白的保守区域。
译  名:
Cloning and Sequence Analysis of AFP Gene Conservative Region in Ammopiptanthus Nanus
作  者:
ZHAN Bin1,2,AN Sha-Zhou1,YANG Li-Juan2,LI Lian-Rui2,MA Chun-Hui2(1.College of Pratacultural and Environmental Science,Xinjiang Agricultural University,Urumqi 830052,China;2.Key Lab.of Science and Technology of Raise Livestock of Tarim,Xinjiang Production and Construction Corps,College of Animal Sciences & Technology,Tarim University,Alaer,Xinjiang 843300,China)
关键词:
Ammopiptanthus nanus;AFP;clone;sequence analysis
摘  要:
【Objective】A pair of primers were designed according to the three antifreezing proteins(AFP) gene of Ammopiptanthus mongolicus(Genbank accession number is AY590122,AY843521,and AY843522,respectively).【Method】Total RNA of Ammopiptanthus nanus was extracted using Trizol,which then was used as a template to amplify conservative region of AFP gene by RT-PCR in Ammopiptanthus nanus.【Result】The result showed that the conservative region of AFP gene of Ammopiptanthus nanus had high homology and similarity(98.15%) to the AFP gene of Ammopiptanthus mongolicus.【Conclusion】The above result could also set the base for the complete AFP gene of Ammopiptanthus nanus

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