作 者：
郭海英;沈文;陈冬梅;陈凯丽;张晓丽;高宝德;孙延鸣
单 位：
石河子大学动物科技学院;石河子大学生命科学学院
关键词：
杀菌/通透性增加蛋白;杂交羊;绵羊肺炎支原体;细胞因子;病理评分
摘 要：
研究重组BPI蛋白(rBPI)对感染绵羊肺炎支原体(MO)的盘羊杂交羊的免疫应答分析。将6只巴什拜羊作为A组,将12只盘羊杂交羊随机分为B、C组,全部人工感染MO,感染第4天开始,C组每天分别注射rBPI,A和B组注射生理盐水。采用ELISA方法检测试验羊不同时间血清中IL-8、IL-10、IFN-γ及TNF-α的含量。在试验结束后宰杀各组6只羊,取肺组织做病理切片,进行治疗效果评价。结果:在感染初期,3个组IL-8、TNF-α及IFN-γ含量都升高,而IL-10有降低趋势。在感染后第7天,C组IL-8浓度显著低于A组(P<0.05);在第14—21天,B组极显著高于A组(P<0.01)。在第5天,C组IL-10显著高于A组(P<0.05),极显著低于B组(P<0.01);在第14天,C组极显著低于A组(P<0.01),显著高于B组(P<0.05);在第21天,C组显著低于A组(P<0.05),极显著高于B组(P<0.01)。在第14天,C组IFN-γ含量显著高于A组(P<0.05);在第21天,C组显著低于B组(P<0.05),但极显著高于A组(P<0.01)。在感染第14天,C组TNF-α显著低于B组(P<0.05),显著高于A组(P<0.05);在第21天,C组的TNF-α显著低于B组(P<0.05),但极显著高于A组(P<0.01)。组织病理学评分结果:A组平均分为9.4,B组平均分19.9,C组平均分为12.8,B组评分显著高于A、C组(P<0.05、P<0.05)。rBPI对MO感染的杂交羊有治疗作用,对细胞因子有调节作用。
译 名：
The Influence of BPI Protein on Cytokines Level in Argali Hybrid Sheep Infected with Mycoplasma Ovipneumoniae
作 者：
GUO Hai-ying;SHEN Wen;CHEN Dong-mei;CHEN Kai-li;ZHANG Xiao-li;GAO Bao-de;SUN Yan-ming;College of Animal Science and Technology,Shihezi University;College of Life Science,Shihezi University;
关键词：
bactericidal/permeability-increasing protein;;argali hybrid sheep;;Mycoplasma ovipneumoniae;;cytokines;;histopathological score
摘 要：
The aim of this study was to analyze the immunological response of the recombinant bactericidal/permeability-increasing protein(rBPI)in Argali Hybrid Sheep(AHS)infected with Mycoplasma ovipneumoniae(MO).Six Bashibai Sheep(BS)was taken as group A,while 12 AHS were randomly divided into group B and C.All the sheep were artificially infected with MO.From the 4th day after infection,group C was treated with rBPI protein,150mg·sheep-1·day-1,and group A and B with the same dose of placebo(Normal saline).The concentrations of IL-8,IL-10,TNF-αand IFN-γfrom serum at different times were detected by ELISA.At the end of the test,all the sheep was slaughtered,and the therapeutic effect was evaluated with pathological section of their lung tissue.Results were as follows:In the early phase of infection,the levels of IL-8,TNF-α,IFN-γwere elevated,while IL-10 revealed a lower trend.On the 7th day post-infection(DPI),the concentration of IL-8in group C was lower than group A(P<0.05).From the 7th to 21 st DPI,IL-8in group B was significantly higher than group A(P<0.01).On the 5th DPI,IL-10 in group C was higher than group A(P<0.05),but lower than group B(P<0.01).On the 14 th to21st DPI,IL-10 in group C was lower than group A(P<0.05),but higher than group B(P<0.05).On the 14 th to 21 st DPI,IFN-γin group C was higher than group A(P<0.05).On the14 th to 21 st DPI,TNF-αin group C was higher than group A(P<0.05,P<0.01),and lower than group B(P<0.05).Histopathological scores of the A,B,C group were 9.4,19.9and 12.8,respectively.The score of group B had significant difference with group A and C(P<0.05,P<0.05).The results showed that rBPI has some treatment effect on AHS infected with MO and a regulatory role on serum cytokines.
