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Position: Home > Articles > Quantitative Analysis of Carbosulfan and Its Degradation Product by HPLC Agrochemicals 2009,48 (9) 36-37+39

高效液相色谱法分析丁硫克百威及其降解产物

作  者:
侯宪文;李勤奋;邓晓;李光义;黄梁财
单  位:
中国热带农业科学院环境与植物保护研究所
关键词:
丁硫克百威;降解产物;高效液相色谱;定量分析
摘  要:
建立丁硫克百威及其降解产物(克百威、3-羟基克百威)的高效液相色谱分析方法。液相色谱条件:流动相为甲醇-水(体积比50:50),流速1.0mL/min,C18柱,紫外检测器波长269nm,进样体积10.0μL。利用峰高进行外标法定量分析,线性相关系数大于0.999,检出限5.5~18.2μg/L。丁硫克百威、克百威和3-羟基克百威峰高值的变异系数分别为0.45%、0.86%、1.11%,回收率分别为102.4%~103.3%、106.9%~108.4%、102.6%~104.7%。丁硫克百威及其降解产物可同时检测和定量分析。
译  名:
Quantitative Analysis of Carbosulfan and Its Degradation Product by HPLC
作  者:
HOU Xian-wen, LI Qin-fen, DENG Xiao, LI Guang-yi, HUANG Liang-cai (environment and Plant Protection research institute, chinese Academy of tropical Agriculture sciences, Danzhou 571737, hainan, china)
关键词:
carbosulfan; degradation product; HPLC; quantitative analysis
摘  要:
A method for quantitative analysis of carbosulfan and its degradation product (carbofuran, 3-hydroxy carbofuran) by HPLC analysis was established. Liquid chromatography conditions: mobile phase was methanol- water(50:50, by vol) at a flow rate of 1.0 ml/min; c18 column; UV detector wavelength: 269 nm; injection volume: 10.0 μL. Use of external standard peak height for the quantitative analysis, linear correlation coefficient greater than 0.999, and the detection limit 5.5-18.2 μg/l. the variation coefficient of peak value for carbosulfan, carbofuran and 3-hydroxy carbofuran were 0.45, 0.86 and 1.11%, separately. the reclaim ratios were between 102.4-103.3%, 106.9-108.4%, 102.6-104.7%. results indicate the carbosulfan and its degradation products at the same time detection and quantitative analysis.

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