中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 纳米ZnO和常规ZnO、ZnSO_4对斑马鱼毒性效应的比较 水产学报 2018 (11) 1804-1816
Position: Home > Articles > Comparative toxicity effect of nano-ZnO, bulk-ZnO, and ZnSO_4 on oxidative stress in zebrafish(Danio rerio) Journal of Fisheries of China 2018 (11) 1804-1816

纳米ZnO和常规ZnO、ZnSO_4对斑马鱼毒性效应的比较

作  者：
兰丽贞;刘林;马宁宁;叶庄新;赵群芬
关键词：
斑马鱼;纳米ZnO;常规ZnO;ZnSO4;毒性效应
摘  要：
为比较纳米ZnO、常规ZnO和ZnSO_4对斑马鱼氧化应激毒性的强弱,探究纳米ZnO的毒性作用与其释放的Zn2+和本身特性的关系,研究了纳米ZnO、常规ZnO、ZnSO_4对斑马鱼肝脏、肠、鳃组织中抗氧化酶活性及炎症凋亡基因表达的影响。实验将斑马鱼分别暴露于纳米ZnO、常规ZnO、ZnSO_4水体中,在4、24和96 h后,用分光光度法检测斑马鱼肝脏、肠、鳃中过氧化氢酶(CAT)、谷胱甘肽(GSH)、谷胱甘肽硫转移酶(GST)、活性氧自由基(ROS)的变化;采用荧光定量PCR技术,测定实验组中肝脏、肠和鳃中Bax、Bcl-2、TNF-α及IL-6的mRNA相对表达量。结果显示,纳米ZnO、常规ZnO、ZnSO_4均引起斑马鱼各组织的氧化应激反应,且使组织中凋亡基因和炎症基因表达水平发生变化,激活生物体内的细胞坏死和细胞凋亡途径,引起细胞死亡或机体炎症,其中纳米ZnO的致氧化损伤作用最强。研究表明,纳米ZnO对斑马鱼的氧化应激毒性强于常规ZnO和ZnSO_4,而纳米颗粒本身特性是导致纳米ZnO毒性作用的主要原因。
译  名：
Comparative toxicity effect of nano-ZnO, bulk-ZnO, and ZnSO_4 on oxidative stress in zebrafish(Danio rerio)
作  者：
LAN Lizhen;LIU Lin;MA Ningning;YE Zhuangxin;ZHAO Qunfen;College of Marine Sciences, Ningbo University;
单  位：
LAN Lizhen%LIU Lin%MA Ningning%YE Zhuangxin%ZHAO Qunfen%College of Marine Sciences, Ningbo University
关键词：
Danio rerio;;nano-ZnO;;bulk-ZnO;;ZnSO4;;toxicity effect
摘  要：
In order to explore the toxicity of nano ZnO and its relationship with the release of Zn2+ and its own characteristics, zebrafish were exposed to the nano-ZnO, bulk-ZnO, and ZnSO_4 suspension for 4, 24 and 96 hours by using the acute toxicity test, and the glutathione-s-transferase(GST), catalase(CAT), and the levels of glutathione(GSH) and reactive oxygen species(ROS), the relative expression levels of Bax, Bcl-2, TNF-alpha and IL-6 in liver, intestine and gill of zebrafish were measured. The objective of this study was to compare the the strength of oxidative stress effects of nano-ZnO, bulk-ZnO and ZnSO_4 in zebrafish, and discuss whether the toxicity of nano-ZnO is related to the releasing Zn2+ or nanoparticles itself. Results showed that nano-ZnO, bulk-ZnO, and ZnSO_4 can cause oxidative stress in the tissues of zebrafish, and the nano-ZnO induced more oxidative damage than the other two forms of zinc. Consequently, we can conclude that the toxicity of nano-ZnO was higher than that of bulk-ZnO and ZnSO_4 on oxidative stress in zebrafish and the toxicity of nano-ZnO was mainly attributed to the nanoparticles itself.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46