当前位置: 首页 > 文章 > 鲤和团头鲂幼鱼卵黄蛋白原的诱导、纯化及电泳比较 水生生物学报 2002,26 (4) 317-321
Position: Home > Articles > INDUCTION,PURIFICATION AND ELECTROPHORETIC COMPARISON OF VITELLOGENIN FROM TWO CYPRINID FISH SPECIES:CARP (CYPRINUS CARPIO) AND WUCHANGFISH (MEGALOBRAMA AMBLYCEPHALA YIH) Acta Hydrobiologica Sinica 2002,26 (4) 317-321

鲤和团头鲂幼鱼卵黄蛋白原的诱导、纯化及电泳比较

作  者:
梁勇;徐盈;杨方星;王剑伟
单  位:
中国科学院水生生物研究所
关键词:
卵黄蛋白原;鲤科鱼类;阴离子交换;内分泌干扰物
摘  要:
采用腹腔注射人工雌激素 1 7α 乙炔基雌二醇 (1 7α ethinyloestradiol,EE2 )的方法 ,在鲤幼鱼和团头鲂幼鱼体内诱导生成卵黄蛋白原 ;并结合利用阴离子交换介质DEAE SephraroseCL 6B和液相层析技术分离纯化了诱导后的鲤幼鱼和团头鲂幼鱼血浆中的卵黄蛋白原 ;SDS PAGE电泳结果表明所分离的两种鲤科鱼类卵黄蛋白原其亚基的分子量分别为 1 70KD和1 5 0KD。
译  名:
INDUCTION,PURIFICATION AND ELECTROPHORETIC COMPARISON OF VITELLOGENIN FROM TWO CYPRINID FISH SPECIES:CARP (CYPRINUS CARPIO) AND WUCHANGFISH (MEGALOBRAMA AMBLYCEPHALA YIH)
作  者:
LIANG Yong, XU Ying, YANG Fang-xing and WANG Jian-wei (Institute of Hydrobiology, The Chinese Academy of Sciences; State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan 430072)
关键词:
Vitellogenin; Cyprinid fish; Anion-exchanger; Endocrine-disrupting chemicals
摘  要:
Vitellogenin was induced in immature common carp (Cyprinus carpio L.) and immature Wuchangfish (Megalobrama amblycephala Yih) by intraperitoneal injection of xenobiotic estrogen 17α-ethinyloestradiol. By using the anion-exchange chromatography on a DEAE-Sephrarose CL-6B column, the vitellogenin in plasma from two cyprinid fish species was effectively purified. The results of SDS-PAGE show that the molecular weights of vitellogenin monomer in two cyprinid fish species are around 170KD and 150KD respectively.

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