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Position: Home > Articles > Microfloral Change of Vacuum-packaged Pork during Chilled Storage FOOD SCIENCE 2011,32 (4) 241-245

真空包装冷却猪肉冷藏过程中菌相变化

作  者:
江芸;高峰;徐幸莲;叶可萍;周光宏
单  位:
南京农业大学国家肉品质量安全控制工程技术研究中心
关键词:
冷却猪肉;真空包装;菌相组成;聚合酶链式反应-变性梯度凝胶电脉法(PCR-DGGE)
摘  要:
应用传统微生物培养和PCR-DGGE方法研究真空包装冷却猪肉4℃贮藏过程中的菌相变化。细菌培养计数结果表明,乳酸菌生长迅速,在贮藏后期即超过了细菌总数值。DGGE结合16S rRNA基因序列分析结果表明,贮藏初期肉中初始菌相较复杂,贮藏末期主要是漫游球菌、肉食杆菌、乳杆菌、乳球菌和热死环丝菌成为优势腐败菌。
译  名:
Microfloral Change of Vacuum-packaged Pork during Chilled Storage
作  者:
JIANG Yun1,2,GAO Feng1,XU Xing-lian1,YE Ke-ping1,ZHOU Guang-hong1 (1. National Center of Meat Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China; 2. Ginling College,Nanjing Normal University,Nanjing 210097,China)
关键词:
chilled pork;vacuum package;microfloral composition;polymerase chain reaction-denaturing gradient gel eletrophoresis (PCR-DGGE)
摘  要:
The microfloral change in vacuum-packaged pork during 4 ℃ storage was explored by traditional bacterial cultivation and PCR-denaturing gradient gel eletrophoresis (PCR-DGGE). Results indicated that lactic acid bacteria (LAB) exhibited a rapid increase and excessive total bacterial counts in the late stage during chilled storage. DGGE combined with the analysis of 16S rRNA gene sequence revealed a complex microflora in chilled pork at the early stage during chilled storage; in contrast,Vagococcus,Carnobacterium,Lactobacillus and Lactococcus,and Brochothrix thermosphacta were the predominant spoilage bacteria at the end of storage period.

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