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当前位置: 首页 > 文章 > UV-B对紫球藻生长抑制及生理特性的影响 水生态学杂志 2018 (6) 114-120
Position: Home > Articles > Effects of UV-B Radiation on the Growth and Physiological Properties of Porphyridium purpureum Journal of Hydroecology 2018 (6) 114-120

UV-B对紫球藻生长抑制及生理特性的影响

作  者：
王静;郭照冰;王瑾瑾;韩珣
单  位：
南京信息工程大学环境科学与工程学院
关键词：
UV-B;紫球藻;抑制作用;生理特性
摘  要：
为了探究紫外线B波段(UV-B)辐照对紫球藻(Porphyridium purpureum)细胞的作用机理,量化胁迫抑制剂量,通过考察紫球藻细胞生长对不同辐照剂量、pH值及培养基的响应,结合紫球藻细胞叶绿素a(Chl-a)、藻红蛋白(PE)、多糖、多不饱和脂肪酸-二十碳五烯酸(EPA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)及丙二醛(MDA)生成量的变化情况,对比分析了其对紫球藻细胞内含物及细胞代谢活性的持续性影响。结果表明,高剂量的UV-B辐照对紫球藻细胞生长有更为明显的抑制作用,辐照剂量大于300mJ/cm2时会直接导致藻细胞群体的大量死亡,抑制并破坏紫球藻细胞内光合色素和藻红蛋白的合成。碱性环境和培养基本身对UV-B辐照抑制紫球藻细胞的生长有一定阻碍。紫球藻细胞内抗氧化酶(SOD、POD、CAT)活性随UV-B辐照剂量的增加先升高后降低,MDA则持续增加。EPA的生成量随UV-B辐照剂量的增大而减少,低剂量的UV-B辐照(75～150mJ/cm2)可促进紫球藻胞外多糖的合成。研究结果可为紫球藻生物活性物质及其衍生物的生产应用提供理论依据,也在一定程度上确立了利用UV-B辐照技术控制紫球藻藻华的工艺条件。
译  名：
Effects of UV-B Radiation on the Growth and Physiological Properties of Porphyridium purpureum
作  者：
WANG Jing;GUO Zhao-bing;WANG Jin-jin;HAN Xun;School of Environmental Science and Engineering,Nanjing University of Information Science & Technology;
关键词：
UV-B;;Porphyridium purpureum;;inhibition;;physiological characteristics
摘  要：
The depletion of atmospheric ozone by chlorofluorocarbons(CFCs)has increased ground level radiation in the UV-B band(280-315 nm).The increased UV-B radiation has affected primary production in microalgae and altered marine ecology.The purpose of this study was to explore the inhibition mechanism of UV-B radiation on P.purpureumgrowth at different UV-B intensities.The effects of UV-B dose,solution pH,and growth media on cell composition and metabolism of P.purpureum were investigated by monitoring cell content of chlorophyll-a(Chl-a),phycoerythrin(PE),extracellular polysaccharides,eicosapentaenoic acid(EPA),superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and malondialdehyde(MDA).The UV radiation source was a collimated UV beam apparatus with a fixed radiant intensity of 0.35 W/m2.The experimental UV treatment doses were achieved by controlling the irradiation time at the fixed intensity,with irradiation times of 0,36,72,96,120,144,192 and 240 min producing corresponding UV doses of 0,75,150,200,250,300,400 and 500 mJ/cm2.The highest UV-B dose had an obvious inhibitory effect on P.purpureum development.Algal cells died quickly at UV doses above300 mJ/cm~2,which caused cell damage and inhibited synthesis of chlorophyll and phycoerythrin.Alkaline conditions and higher media nutrient levels reduced growth suppression of P.purpureum by UV-B irradiation to a limited extent.Although MDA rose continuously with increasing UV-B dose,antioxidant enzyme activities(SOD,POD and CAT)initially increased and then decreased with dose.Further,increased UVB dose decreased cell EPA content and low UV-B doses(75-150 mJ/cm~2)increased production of extracellular polysaccharide.The study provides a theoretical foundation for the production of biological active substances by P.purpureum and establishes conditions for controlling algal blooms by UV-B irradiation.

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