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当前位置: 首页 > 文章 > 烟夜蛾性肽受体基因的克隆与表达模式分析 昆虫学报 2014,57 (11) 1281-1288
Position: Home > Articles > Molecular cloning and expression pattern analysis of sex peptide receptor gene in Helicoverpa assulta (Lepidoptera: Noctuidae) Acta Entomologica Sinica 2014,57 (11) 1281-1288

烟夜蛾性肽受体基因的克隆与表达模式分析

作  者：
范荫荫;安世恒;王琼;刘孝明;李为争;郭线茹;原国辉
单  位：
河南农业大学植物保护学院
关键词：
烟夜蛾;性肽;性肽受体;性信息素腺体;基因表达模式;交配
摘  要：
【目的】克隆烟夜蛾Helicoverpa assulta(Guenée)性肽受体基因并分析其表达模式,为深入研究性肽与交配后反应的关系奠定基础。【方法】采用RT-PCR方法,从烟夜蛾雌蛾性信息素腺体中得到性肽受体基因c DNA全序列。利用荧光定量PCR方法,分析该基因的表达模式。【结果】序列分析结果显示,烟夜蛾性肽受体基因c DNA全长2 048 bp,命名为Hass SPR(Gen Bank登录号:AFH53182.1)。该基因的开放阅读框长1 275 bp,编码424个氨基酸残基,序列中含有7个跨膜域结构,预测分子量和等电点分别为48.6 k Da和9.25。序列比对分析表明,Hass SPR与近缘种棉铃虫H.armigera和其他蛾类性肽受体的氨基酸序列一致性分别达98.35%和超过84%,与已经报道的其他昆虫的性肽受体的氨基酸序列一致性也在64%以上。不同组织表达分析表明,Hass SPR在测定的1日龄雌蛾不同组织中均有表达,以在脑中的表达量最高。时序表达分析表明,在羽化前1天至羽化后6日龄雌蛾的信息素腺体中均有表达,以3日龄表达量最高。雌蛾交配后,Hass SPR在性信息素腺体和脑中的表达量显著上调,而在交配囊和卵巢中的表达量显著下调。【结论】从烟夜蛾雌蛾性信息素腺体中克隆得到性肽受体基因Hass SPR,其表达模式提示该基因的表达水平与雌蛾的生殖生理和生殖行为有关。
译  名：
Molecular cloning and expression pattern analysis of sex peptide receptor gene in Helicoverpa assulta (Lepidoptera: Noctuidae)
作  者：
FAN Yin-Yin;AN Shi-Heng;WANG Qiong;LIU Xiao-Ming;LI Wei-Zheng;GUO Xian-Ru;YUAN Guo-Hui;College of Plant Protection,Henan Agricultural University;
关键词：
Helicoverpa assulta;;sex peptide;;sex peptide receptor;;sex pheromone gland;;gene expression pattern;;mating
摘  要：
【Aim 】 In order to explore the relationship between sex peptide( SP) and post-mating response( PMR),the sex peptide receptor( SPR) gene from Helicoverpa assulta( Guenée) was cloned and its expression patterns were analyzed. 【Methods】The full-length c DNA sequence of SPR gene was cloned from sex pheromone glands of H. assulta by RT-PCR method. The expression patterns of SPR gene were analyzed by using qRT-PCR. 【Results】The full-length c DNA sequence of SPR gene from H.assulta is 2 048 bp in size and named as Hass SPR( Gen Bank accession number: AFH53182. 1). The open reading frame of Hass SPR is 1 275 bp that encodes 424 amino acid residues with 7 putative transmembrane domains. The predicted molecular weight and isoelectric point of Hass SPR are 48. 6 k Da and 9. 25,respectively. The alignment analysis indicated that Hass SPR shared 98. 35% and over 84%amino acid sequence identities with SPR proteins from its close relative species H. armigera and other moths,respectively,and shared high identities( > 64%) with the corresponding SPR proteins from other insects. The tissue expression profiles revealed that Hass SPR could be detected in all tested tissues of 1day-old female adults of H. assulta,and had the highest expression level in brain. The temporal expression profiles showed that the Hass SPR was expressed in female pheromone gland from 1 d before emergence to 6 d after emergence and reached the peak at 3 d after emergence. After mating,the expression level of Hass SPR in female brain and pheromone gland significantly increased,while that in bursa copulatrix and ovary significantly decreased. 【Conclusion】The sex peptide receptor gene Hass SPR was cloned from sex pheromone glands of H. assulta. The expression level of Hass SPR is associated with the reproductive physiology and reproductive behavior of female adults of this moth.

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