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Position: Home > Articles > Preparation and Immune Efficacy of Salmonella choleraesuis Ghost Chinese Journal of Veterinary Drug 2016,50 (9) 22-25

猪霍乱沙门菌菌蜕的制备及免疫保护效力研究

作  者:
单晓枫;陈龙;李茂辉;康元环;孙武文;张伟;钱爱东
单  位:
吉林农业大学动物科学技术学院
关键词:
猪霍乱沙门菌;菌蜕;免疫保护效力;裂解基因E;裂解效率
摘  要:
为制备猪霍乱沙门菌菌蜕并研究其免疫保护效力,克隆噬菌体phi X174裂解基因E,与p BV220连接,构建温控溶菌表达载体,将该载体转入猪霍乱沙门菌TTB1中,制备菌蜕并对其裂解率、安全性以及对小鼠的免疫保护效力进行了研究。结果显示:成功克隆了裂解基因E、片段大小274 bp,构建了温控溶菌质粒载体p BVE,制备了猪霍乱沙门菌菌蜕,其裂解率最高为99.46%、经冻干灭活残余活菌,安全性试验检测证实其安全后,对小鼠进行了免疫保护试验,其保护率为70%、与弗氏佐剂灭活苗保护率相当、优于甲醛灭活苗。研究为猪霍乱沙门菌感染的防治奠定了基础。
译  名:
Preparation and Immune Efficacy of Salmonella choleraesuis Ghost
作  者:
SHAN Xiao-feng;CHEN Long;LI Mao-hui;KANG Yuan-huan;SUN Wu-wen;ZHANG Wei;QIAN Ai-dong;College of Animal Science and Technology,Jilin Agricultural University;
关键词:
Salmonella choleraesuis;;bacterial ghost;;protective immunity;;lysis gene E;;lysis efficiency
摘  要:
In order to develop a Salmonella choleraesuis bacterial ghost vaccine and investigate its protective immunity. This study,we cloned the lysis gene E from phage phi X174 and inserted into an temperature-induced expression plasmid p BV220 vector,and then wastransformed into Salmonella TTB1. We created the Salmonella choleraesuis ghost and studied its lytic efficiency and protective immunity. The results showed that the lysisgene E about 274 bp was clone,the temperature-induced lytic plasmid p BVE and Salmonella choleraesuis bacterial ghost were created. The lysis rate can be 99. 46% and no life bacteria survived after freeze drying,The results of protective immunity showed that Salmonella ghost group providethe protection rate of 70%,The protection rate ofplasmid-type Salmonella ghost vaccine strain was comparable with that of Salmonella inactivated vaccine with adjuvant,which was better than that of the inactivated vaccine. The research provides a foundation for the prevention and treatment of diseases caused by Salmonella choleraesuis.

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