作 者：
贾知锋;王纯洁;敖日格乐;高瑞娟;巴森胡
单 位：
内蒙古农业大学兽医学院;内蒙古农业大学动物科学学院
关键词：
nisin;传统蒙药复方;神经递质;应激性腹泻;肠道菌群;脑-肠轴
摘 要：
旨在研究乳酸链球菌素(nisin)对应激性腹泻导致的肠道菌群与脑-肠轴功能之间的相关性,进一步揭示nisin对单胺类神经递质的调控机制。通过注入3种血清型大肠杆菌混合菌悬液(1∶1,1.0×109 CFU·mL-1)制备小鼠腹泻模型,并用nisin和蒙药复方进行治疗;在造模后0、72h,ELISA检测5-HT、DA和NE水平,以及平板涂布法将盲肠内容物分别10倍稀释成8个梯度涂布于相应培养基,计算菌落数。结果表明,(1)在脑组织中,造模后72h时nisin、传统蒙药复方、成分复方、抗生素组中5-HT分别升高到0h时的108.60%、124.04%、117.47%、115.57%;并且使十二指肠中5-HT分别增加到0h时的289.18%、200.30%、271.59%,nisin组显著高于其他各组(P<0.05);造模后除空肠外,72h与0h相比,nisin和传统蒙药复方对小鼠血清、脑、十二指肠、回肠以及结肠中5-HT都有显著提升作用(P<0.05);(2)造模后72h时,nisin、蒙药复方、成分复方及抗生素可提高正常小鼠脑、肠中多巴胺,并以回肠中增加最多,且差异显著(P<0.05);(3)在脑组织中0h与72h时NE的浓度,传统蒙药复方、nisin分别升高到115.62%和106.17%,且均与空白、阴性对照组相比差异显著(P<0.05);在十二指肠、回肠中,nisin组72h比0h升高了170.97%、121.74%,尤其十二指肠明显高于其他各组且差异显著(P<0.05);(4)nisin、有效成分复方和传统蒙药复方可显著提高感染E.coli小鼠肠道内有益菌乳酸菌和双歧杆菌数量,显著降低有害菌肠杆菌和肠球菌数量(P<0.05)。可见,nisin和蒙药复方均显著提高细菌感染小鼠血液、脑和各肠段中5-HT、NE含量,尤以十二指肠突出,nisin和传统蒙药能打破肠道中5-HT的平衡,并伴随着肠道各段、血液中5-HT的升高脑组织中5-HT含量也增加。
译 名：
Effect of Nisin on Monoamine Neurotransmitters of Mice with Diarrhea Induced by Escherichia coli
作 者：
JIA Zhi-feng;WANG Chun-jie;AO-Ri-ge-le;GAO Rui-juan;BA Sen-hu;College of Veterinary Medicine,Inner Mongolia Agricultural University;College of animal science,Inner Mongolia Agricultural University;
关键词：
nisin;;Mongolian medicine;;neurotransmitter;;stress-diarrhea;;intestinal flora
摘 要：
The paper aims to study the correlation between Streptococcus thermophilus and intestinal flora and brain-gut axis function during diarrhea irritable,and further reveals the regulation mechanism of nisin on monoamine neurotransmitters.By injecting three serotypes of Escherichia coli mixed bacterium suspension liquid(1∶1,1.0×109 CFU· mL-1),the diarrhea model of mice was prepared,and nisin and Mongolian medicine were used for treatment.Tissus samples were collected at 0h,72 hpost modeling and ELISA kits were used to detect the levels of 5-HT,DA and NE,as well as the caecum contents plate were diluted 10 fold into eight gradient coating on the responging medium to calculate bacterial colony number by using plat coating method.The re-sults showed that:(1)In brain tissue,5-HT level at 72 hpost modeling in the nisin,traditional Mongolian medicine(TMM)compound,TMM ingredients,and antibiotic groups were increased to108.60%,124.04%,117.47%,115.57% of 0h,respectively;and the duodenal 5-HT in the nisin,TMM compound and TMM ingredients groups at 72 hpost modeling were increased to289.18%,200.30%,271.59% of 0h,respectively,nisin group was significantly higher than other groups(P<0.05).After modeling,in addition to the jejunum,compared 72h's with 0h's,nisin and TMM compound have signicantly improved 5-HT level(P<0.05)in mice serum,brain,duodenum,ileum and colon.(2)After 72 h,nisin,TMM compound,TMM ingredients and antibiotic can improve the dopamine in the intestine and brain of normal mice,and that in the ileum increased the most,and the difference was significant(P<0.05).(3)In brain tissue at 0hand 72 h,NE concentration increased to 115.62%and 106.17%,respectively under the react of TMM compound and nisin,and which also have significant difference comparing with blank control,and the negative control group(P<0.05);in the duodenum and ileum,the EN concentration of nisin group at by 72 h were increased by 170.97%,121.74%,especially that in the duodenum was higher than other groups and the difference was significant(P<0.05).(4)Nisin,TMM effective ingredients and TMM compound can significantly increase the number of intestinal probiotic Lactobacillus and Bifidobacteriumin E.coli infected mice,and significantly reduce the number of harmful bacteria Enterobacter and Enterococcus(P<0.05).In addition,nisin and TMM compound were significantly increased bacterial infections in mice blood,brain and various intestinal segments in 5-HT,NE content,especially in the duodenum,nisin and traditional Mongolian medicine can break the balance of intestinal 5-HT,and accompanied by increase of 5-HT in the intestine and blood,the brain 5-HT content also increased.
