作 者：
董玲;郑明明;郭萍梅;李文林;邓乾春;黄凤洪;郑畅
关键词：
磁性颗粒;酯化反应;固定化脂肪酶;甾醇酯
摘 要：
采用固载有Candida rugosa的磁性固定化酶为生物催化剂,并将其用于α-亚麻酸植物甾醇酯的酶促合成。通过响应面分析优化实验条件,得到最佳工艺参数为:固定化酶用量为25.2mg/mL,甾醇底物浓度为146.5μmol/mL,异辛烷为溶剂,55℃反应5h,得到的植物甾醇的酯化率为92.61%。该固定化脂肪酶经过简单的溶剂清洗可以重复使用10次,仍能保持71.8%的催化活性。
译 名：
Immobilization of Candida rugosa lipase on magnetic polymer microsphere for synthesis of phytosterol esters of alpha-linolienic acid
作 者：
DONG Ling1,2,ZHENG Ming-ming1,GUO Ping-mei1,LI Wen-lin1, Deng Qian-chun1,HUANG Feng-hong1*,ZHENG Chang1(1.Hubei Key Laboratory of Lipid Chemistry and Nutrition,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China; 2.College of Food Science and Technology,Huazhong Agriculture University,Wuhan 430070,China)
关键词：
Magnetic microsphere;Esterification;Immobilization lipase;Phytosterol esters
摘 要：
Immobilized enzyme of Candida rugosa lipase on magnetic microsphere was used as biocatalyst for synthesis of phytosterol esters of alpha-linolienic.Response surface methodology(RSM) was employed to evaluate the effects of synthesis conditions.The optimum synthesis conditions were as follows: 25.2mg/mL immobilized CRL,146.5μmol/mL phytosterols concentration,at 55℃ for 5h in the presence of isooctane,the degree of phytosterols esterification was 92.61%.By solvent cleaning,the immobilization CRL sustained 78.1% activities after having been recycled 10 times.
