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当前位置: 首页 > 文章 > 樟叶越桔愈伤组织诱导及其细胞悬浮培养体系建立 西部林业科学 2019,48 (5) 119-124+130
Position: Home > Articles > Callus Induction and Establishment of Suspension Culture System of Vaccinium dunalianum Journal of West China Forestry Science 2019,48 (5) 119-124+130

樟叶越桔愈伤组织诱导及其细胞悬浮培养体系建立

作  者：
付羚;张訸;罗旭璐;尚俊可;唐军荣;赵平
单  位：
西南林业大学 西南山地森林资源保育与利用教育部重点实验室;西南林业大学 西南地区林业生物质资源高效利用国家林业局重点实验室
关键词：
樟叶越桔;愈伤组织;诱导;增殖;细胞悬浮培养
摘  要：
以樟叶越桔组培苗幼嫩叶片为外植体,研究了植物生长调节剂对其愈伤组织诱导和增殖的影响,建立樟叶越桔的细胞悬浮培养体系。结果表明,樟叶越桔的愈伤组织诱导的适宜培养基为WPM+2.0mg/L 6-BA+0.05mg/L NAA+2.0 mg/L 2,4-D+30%蔗糖。樟叶越桔愈伤组织增殖的最佳植物生长调节剂组合为2.0mg/L ZT+0.4mg/L NAA,得到的蓬松愈伤组织适宜进行其悬浮培养。WPM 3/5有机为适宜的改良培养基,通过细胞悬浮生长曲线的测定,确定樟叶越桔细胞悬浮培养较适宜的继代周期为14d,最长不宜超过21d。
译  名：
Callus Induction and Establishment of Suspension Culture System of Vaccinium dunalianum
作  者：
FU Ling;ZHANG He;LUO Xu-lu;SHANG Jun-ke;TANG Jun-rong;ZHAO Ping;Key Laboratory of State Forestry Administration for Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University;Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China Southwest Forestry University,Ministry of Education;
关键词：
Vaccinium dunalianum;;callus;;induction;;proliferation;;cell suspension culture
摘  要：
Using the young leaves of the Vaccinium dunalianum seedlings as an explant,the effects of plant growth regulators on the induction and proliferation of callus were investigated for the establishment of the cell suspension culture system.The results showed that the optimal medium for callus induction were WPM+2.0 mg/L 6-BA+0.05 mg/L NAA+2.0 mg/L 2,4-D+30% sucrose.The best plant growth regulator combination for callus proliferation was 2.0 mg/L ZT+0.4 mg/L NAA,and the obtained callus was suitable for cell suspension culture.The best improved medium was organic WPM 3/5,and the callus growth curve test showed that the suitable subculture period was 14 d and the longest subculture period was not more than 21 d.

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