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当前位置: 首页 > 文章 > 滇西北深纹核桃(Juglans sigillata)种质资源遗传多样性研究 云南农业大学学报(自然科学) 2018 (4) 597-604
Position: Home > Articles > Study on the Genetic Diversity of Germplasm Resources of Juglans sigillata in Northwest Yunnan Journal of Yunnan Agricultural University(Natural Science) 2018 (4) 597-604

滇西北深纹核桃(Juglans sigillata)种质资源遗传多样性研究

作  者：
肖良俊;陈少瑜;宁德鲁;吴涛;贺娜
关键词：
深纹核桃;种质资源;滇西北;SSR标记;遗传多样性
摘  要：
【目的】揭示滇西北深纹核桃(Juglans sigillata)种质资源的遗传多样性,为该区域深纹核桃资源的有效利用和科学保护提供依据。【方法】采用微卫星(SSR)分子标记技术对滇西北13个群体共332份深纹核桃种质进行了遗传多样性分析;利用分子方差分析(AMOVA)揭示群体间的遗传分化状况;用Mantel检验估算群体间遗传距离和地理距离的相关性。【结果】(1)滇西北13个核桃群体的期望杂合度介于0.456 5~0.630 0之间,平均为0.528 6;Shannon信息指数介于0.852 9~1.336 7之间,平均为1.026 4;Nei’s基因多样性指数在0.442 1~0.622 5之间,平均为0.528 6,遗传多样性属于中等水平,遗传多样性呈由西北群体(迪庆和怒江)向东南群体(丽江和大理)逐步降低的分布格局。(2)群体间的遗传分化系数为0.101 1,遗传变异主要来自群体内;AMOVA分析也得到了类似的结果,群体内的分化是该区域种质资源变异的主要来源。(3) UPGMA聚类结果显示:13个群体在遗传一致度为0.85时被聚成了4组;Mantel检验结果表明:群体间遗传距离与地理距离相关性显著(r=0.644 1,P=0.002 0)。【结论】长期的自然选择及人为影响,导致了滇西北核桃种质资源遗传多样性水平及特有的分布格局,该区域核桃资源群体间的遗传分化较低,遗传变异主要来源于群体内。资源选育和保护时,在选择遗传多样性高的群体基础上,应侧重于群体内家系和单株的选择。
译  名：
Study on the Genetic Diversity of Germplasm Resources of Juglans sigillata in Northwest Yunnan
作  者：
XIAO Liangjun;CHEN Shaoyu;NING Delu;WU Tao;HE Na;Institute of Economic Forest Trees, Yunnan Academy of Forestry;Woody Oil Engineering Research Center of Yunnan Province, Yunnan Academy of Forestry;The Key Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry;
单  位：
XIAO Liangjun%CHEN Shaoyu%NING Delu%WU Tao%HE Na%Institute of Economic Forest Trees, Yunnan Academy of Forestry%Woody Oil Engineering Research Center of Yunnan Province, Yunnan Academy of Forestry%The Key Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry
关键词：
Juglans sigillata;;germplasm resources;;northwest Yunnan;;SSR markers;;genetic diversity
摘  要：
[Purpose]Germplasm resources of Juglans sigillata in northwest Yunnan were studied to reveal genetic diversity and genentic structure and to provide basis for scientific conservation and effective utilization of the genetic resources.[Method]332 samples from 13 walnut populations were taken in the analysis by SSR molecular markers technology. Genetic variance was revealed through AMOVA, and Mantel's test was used to analyze the correlation between genetic distance and geographic distance.[Result](1) The expected heterozygosity ranged from 0.456 5 to 0.630 0, with an average of 0.528 6. The Shannon's information index was between 0.852 9 and 1.336 7, with an average of 1.026 4 and the Nei's gene diversity ranged from 0.442 1 to 0.622 5, with an average of0.528 6. The data suggest a medium genetic diversity in this area. The genetic diversity level showed pattern of reduction from the northwest(Diqing and Nujiang) to the southeast(Lijiang and Dali).(2)The proportion of genetic differentiation among populations was 0.101 1, which means that genetic variation derived mainly from within populations. AMOVA analysis got the same result.(3) Cluster analysis showed 13 populations were divided into 4 groups at genetic identity of 0.85. Mantel's test showed a significant correlation between genetic distance geographic distance(r=0.644 1, P=0.002 0).[Conclusion]Medium genetic diversity and the particular genetic variation patterns of walnut germplasm resources in northwest Yunnan have been developed through long term of natural selection and human activities. The proportion of genetic differentiation among populations was low, showing the genetic variation derived mainly from within populations. For the selection and conservation of resources in the area, it should focus on the selection of families and individuals within populations based on the selection of selection of populations with rich genetic diversity.

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