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Position: Home > Articles > Effect of Chinese Herbal Medicinal Ingredients on IL-2mRNA Levels of T Lymphocytes in Mice Measured with Semi-quantification RT-PCR Scientia Agricultura Sinica 2005,38 (9) 1911-1916

半定量RT-PCR测定中药成分对小鼠脾T细胞IL-2mRNA水平的影响

作  者:
储岳峰;颜新敏;李祥瑞;胡元亮
单  位:
南京农业大学动物医学院;中国农业科学院兰州兽医研究所
关键词:
小鼠;中药成分;半定量RT-PCR;IL-2mRNA;T细胞
摘  要:
应用半定量RT-PCR方法,测定了9种中药成分对小鼠脾脏T细胞IL-2mRNA水平的影响。结果显示,体内注射黄芪多糖、淫羊藿多糖、当归多糖、蜂胶黄酮和黄芪皂甙能够使ConA诱导的小鼠脾T细胞IL-2mRNA水平显著升高,蜂胶多糖、板兰根多糖、人参皂甙、淫羊藿黄酮不能影响细胞内IL-2mRNA丰度;中药成分体外诱导时与体内应用时的作用效应相同,但与对照相比较,蜂胶多糖在体外能够显著促进IL-2mRNA的诱生,与体内不同。
译  名:
Effect of Chinese Herbal Medicinal Ingredients on IL-2mRNA Levels of T Lymphocytes in Mice Measured with Semi-quantification RT-PCR
作  者:
CHU Yue-feng1,2,YAN Xin-min2,LI Xiang-rui1,HU Yuan-liang1(1 College of Veterinary Medicine,Nanjing Agricultural Universtity,Nanjing 210095;2Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046)
关键词:
Mice;Chinese herbal medicinal ingredients;Semi-quantification RT-PCR;IL-2mRNA;T lymphocytes
摘  要:
In this study,the IL-2 mRNA levels of T lymphocytes in normal mice stimulated by nine Chinese herbal medicinal ingredients(CHMIs) were measured with a reverse transcription and semi-quantification polymerase chain reaction.The results showed that astragalus polysaccharide(APS),epimedium polysaccharide(EPS),Chinese angelica polysaccharide(CAPS),propolis flavone(PF)and astrogaloside(AS) promoted IL-2 mRNA levels in T lymphocytes in vitro and in vivo in different degrees,and the level of IL-2 mRNA induced by propolis polysaccharide(PPS) in vitro was higher than that of the control,which differed from that of PPS in vivo.

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