摘 要：
研究荷叶总黄酮(total flavonoids of lotus leaf(Nelumbo nuciferea Gaertn.),TFL)对小鼠酒精肝损伤的影响及作用机理。将72只小鼠随机分为正常对照组、模型组、益肝灵组、TFL低剂量组、TFL中剂量组、TFL高剂量组。除了空白组,用TFL(100、300、500mg/kg)灌胃小鼠,益肝灵组灌胃益肝灵,每日灌胃后4h用50%乙醇12mL/kg·bw灌胃,连续14d,末次乙醇灌胃6h后,处死小鼠,计算脏器指数,检测各实验组小鼠血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)活性、甘油三酯(TG)含量、肝组织匀浆中丙二醛(MDA)、谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)、谷光甘肽过氧化物酶(GSH-Px)活性;酶联免疫法测定肝匀浆中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的含量;对肝进行病理组织学检查。结果表明:荷叶总黄酮能显著降低酒精肝损伤小鼠血清ALT、AST、TG水平,降低肝匀浆MDA含量,抑制SOD、GSH-Px、GSH水平的下降,抑制TNF-α和IL-1β的表达,并减轻酒精导致的肝组织病理损伤,呈现剂量效应关系。说明荷叶总黄酮对酒精致小鼠肝损伤具有较好的保护作用,其机制可能与其清除自由基、抑制脂质过氧化、抑制TNF-α、IL-1β因子的表达有关。
译 名：
Protective effects of total flavonoids of lotus leaf( Nelumbo nuciferea Gaertn.) on alcohol-induced liver injury in mice
作 者：
LIANG Shu-cai;ZONG Zi-wei;YU Hai-ying;LI Xian-jia;Department of Basic Medicine,Luohe Medical College;
关键词：
total flavonoids of lotus leaf(TFL);;liver injury;;alcohol
摘 要：
The present study aimed at evaluating the hepatoprotective effects of total flavonoids of lotus leaf( Nelumbo nuciferea Gaertn..)( TFL) on the injury induced by alcohol in mice.Seventy- two male Kunming mice were randomly divided into control group,model group,Yiganling group and TFL group( 100,300,500mg /kg),12 in each group.Mice were administered orally with 50% alcohol 12mL /kg at 4h after the doses of TFL everyday for fourteen consecutive days except mice in normal group.The protective effect was evaluated by biochemical parameters including serum aspartate transaminase( AST),alanine transferase( ALT),triglyceride( TG) and reduced glutathione( GSH),malondialdehyde( MDA),superoxide dismutase( SOD) and glutathione peroxidase( GSH- Px) in liver tissue.The protective effects on alcohol- induced liver injury of TFL were confirmed by hematoxylin- eosin staining and liver homogenate TNF- α,IL- 1βcontents in liver by ELISA analysis.The results showed: Compared with model group,TFL could significantly reduce serum ALT,AST,TG levels of alcoholic-induced liver injury in mice,lower MDA content in liver homogenates,and inhibit the decrease of SOD,GSH- Px, GSH levels in a dose- dependent manner,and inhibit the expression of TNF- α and IL- 1β in liver tissue,and alleviate liver tissue pathological damage.This study showed that total flavonoids of lotus leaf( TFL) had remarkable protective effect on alcohol- induced hepatic injury in mice,possibly due to its capability of scavenging reactive oxygen species,inhibiting lipid peroxidation and expression of TNF-α and IL-1βcytokines.
