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Position: Home > Articles > Optimization of Fermentation Medium for β-Fructofuranosidase Production from Arthrobacter sp.10138 Using Uniform Design FOOD SCIENCE 2009,30 (19) 218-222

均匀设计优化节杆菌产β-呋喃果糖苷酶发酵培养基

作  者:
代志凯;唐嘉婕;阮征;印遇龙
单  位:
南昌大学食品科学与技术国家重点实验室南昌大学生命科学与食品工程学院;华南理工大学轻工与食品学院;中国科学院亚热带农业生态研究所;南昌大学生命科学与食品工程学院
关键词:
β-呋喃果糖苷酶;低聚乳果糖;均匀设计;培养基优化
摘  要:
采用均匀设计法,优化节杆菌发酵产β-呋喃果糖苷酶培养基。采用6因素10水平混合均匀设计表,综合考察蔗糖、牛肉膏、酵母膏、(NH4)2HPO4、KH2PO4、MgSO4.7H2O6种培养基成分对节杆菌发酵产β-呋喃果糖苷酶转移糖基酶活力的影响。通过二次多项式逐步回归得出最终培养基组成为:蔗糖5g/L、牛肉膏30g/L、酵母膏2g/L、(NH4)2HPO410g/L、KH2PO40.5g/L、MgSO4.7H2O0.2g/L,在此条件下回归方程的酶活力理论值为382.93U/ml,验证实验中酶活力为365.85U/ml,验证值与预测值之间吻合较好。
译  名:
Optimization of Fermentation Medium for β-Fructofuranosidase Production from Arthrobacter sp.10138 Using Uniform Design
作  者:
DAI Zhi-kai1,2,TANG Jia-Jie2,RUAN Zheng1,2,YIN Yu-long1,2,3 (1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;2. College of Life Science and Food Engineering,Nanchang University,Nanchang 330031,China;3. Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China)
关键词:
β-fructofuranosidase;lactosucrose;uniform design method;medium optimization
摘  要:
A uniform design involving 30 experiments of 6 variables at 10 levels was applied to optimize the fermentation medium composition of Arthrobacter sp.10138 for high activity of β-fructofuranosidase. The experimental data were analyzed by stepwise regression to obtain a quadratic polynomial regression model for predicting β-fructofuranosidase activity as a function of 6 variables. The optimal medium composition was as following:sucrose 5 g/L,beef extract 30 g/L,yeast extract 2 g/L,(NH4)2HPO4 10 g/L,KH2PO4 0.5 g/L and MgSO4.7H2O 0.2 g/L. Under these conditions,the predicted maximum β -fructofuranosidase activity was 382.92 U/ml,which was in close agreement with the actual experimental value of 365.85 U/ml.

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