中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 固相萃取联合超高效液相色谱-串联质谱法测定畜肉中3种儿茶酚胺类物质 食品科学 2019 (24) 300-305
Position: Home > Articles > Determination of Three Catecholamines in Livestock Meat by Solid Phase Extraction Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry FOOD SCIENCE 2019 (24) 300-305

固相萃取联合超高效液相色谱-串联质谱法测定畜肉中3种儿茶酚胺类物质

作  者：
任南;赵文涛;陈超;吴彦超;张颖颖;李莹莹;郭文萍;范维;王守伟
单  位：
中国肉类食品综合研究中心
关键词：
儿茶酚胺;畜肉;β受体激动剂;固相萃取技术;超高效液相色谱-串联质谱法
摘  要：
建立超高效液相色谱-串联质谱技术同时检测畜肉中3种儿茶酚胺类物质。畜肉样品用高氯酸溶液提取,经混合阳离子固相萃取小柱净化,HILIC Plus色谱柱(4.6 mm×100 mm,3.5μm)分离,流动相分别为甲酸-水(1∶99,V/V)和乙腈,采用电喷雾离子源,正离子多反应监测,对儿茶酚胺类药物进行检测,外标法实现定量分析。结果表明:3种儿茶酚胺类药物在40～1 000 ng/mL范围内线性良好,相关系数大于0.99,畜肉中检出限(R_(SN)≥3)为10μg/kg,定量限(R_(SN)≥10)为40μg/kg;添加量为40～1 000μg/kg的3种儿茶酚胺类物质的回收率均在75.48%～91.25%之间,方法的精密度在1.2%～4.6%之间(n=6)。该方法前处理简单、快速、准确,为畜肉中儿茶酚胺的检测提供方法。
译  名：
Determination of Three Catecholamines in Livestock Meat by Solid Phase Extraction Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
作  者：
REN Nan;ZHAO Wentao;CHEN Chao;WU Yanchao;ZHANG Yingying;LI Yingying;GUO Wenping;FAN Wei;WANG Shouwei;China Meat Food Research Center;
关键词：
catecholamines;;livestock meat;;β adrenergic agonist;;solid phase extraction;;ultra-high performance liquid chromatography-tandem mass spectrometry
摘  要：
This study aimed to establish a method for simultaneous qualitative and quantitative analysis of three catecholamines(CAs) in livestock meat by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLCMS/MS). The samples were homogenized, extracted with perchloric acid solution, and purified on a PCX solid phase extraction cartridge. The chromatographic separation was achieved on an HILIC Plus column(4.6 mm × 100 mm, 3.5 μm) using a mobile phase consisting of acetonitrile and formic acid in water(1:99, V/V). The analytes were ionized by positive electrospray(ESI+), detected in the multiple reaction monitoring mode(MRM) and quantified by the external standard method. The results indicated that a good linearity over the concentration range of 40–1 000 ng/mL was observed for all the analytes with correlation coefficients greater than 0.99. The limits of detection(LOD) and quantification(LOQ) were 10 and 40 μg/kg, respectively. The average recoveries of the CAs ranged from 75.48% to 91.25% with relative standard deviations(RSDs) of 1.2%–4.6%(n = 6). The method is sensitive, simple, rapid and accurate and can meet the requirements for practical analysis.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46