作 者：
李小杰;李淑君;陈玉国;王海涛;李成军;赵凤霞;李彦平
关键词：
豫南烟区;烟草青枯病菌;致病力;遗传多样性
摘 要：
为有效防控豫南烟区烟草青枯病,对来自信阳市罗山县、驻马店市确山县和遂平县的烟草青枯病菌的致病力和遗传多样性进行了测定分析。人工接种试验表明,豫南烟区烟草青枯病菌的致病力分为强、中和弱3种,不同寄主来源的烟草青枯病菌具有显著的致病力差异。从100个随机引物中筛选出8个扩增条带清晰且具有丰富多态性的引物用于不同地区烟草青枯病菌菌株DNA的RAPD分析,共扩增出720条带,其中多态性带占88.9%。聚类结果和遗传相似系数分析显示,供试菌株可分为A、B两大类,遗传相似系数范围为0.49~1.00,且供试菌株间的遗传相似性与其地理来源有一定的相关性。综上所述,豫南烟区烟草青枯病菌的致病力存在明显差异且其DNA具有丰富的遗传多样性。
译 名：
Analysis of Pathogenicity and Genetic Diversity of Ralstonia Solanacearum on Tobacco in Southern Henan Province
作 者：
LI Xiaojie;LI Shujun;CHEN Yuguo;WANG Haitao;LI Chengjun;ZHAO Fengxia;LI Yanping;Tobacco Research Institute of Henan Academy of Agricultural Sciences;
关键词：
tobacco area in southen Henan province;;tobacco bacterial wilt;;differentiation of pathogenicity;;genetic diversity
摘 要：
In order to take effective measures to control tobacco bacterial wilt in Henan Province, the pathogenicity and genetic diversity of Ralstonia solanacearum from tobacco in Luoshan, Queshan and Suiping counties of Henan Province were investigated. The results showed that all the isolates could be divided into three pathogenic types including strong, medium and week pathogenicity by artificial inoculation. Also, the R. solanacearum strains from different hosts showed significant difference in pathogenicity. Eight primers which were screened from 100 primers were used in RAPD analysis of R. solanacearum Isolates. A total of 720 bands were obtained and 88.9% of them were polymorphic. Based on clustering and similarity analysis, the tested isolates fell into two groups and the genetic similarity among different strains was from 0.49 to 1.00. Moreover, the genetic similarity might be related to different geographical origin. In summary, the R. solanacearum isolates infecting tobacco in southern Henan Province showed obvious differentiation of pathogenicity with abundant genetic diversity.
