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Position: Home > Articles > Cloning and sequence analysis of gam22 gene from Eimeria necatrix Animal Husbandry & Veterinary Medicine 2014,46 (5) 10-13

毒害艾美耳球虫配子体gam22基因的克隆与序列分析

作  者:
刘丹丹;曹李琴;朱玉兰;许金俊;吴力力;金文杰;陶建平
单  位:
江苏省动物重要疫病与人兽共患病防控协同创新中心;扬州大学兽医学院
关键词:
毒害艾美耳球虫;gam22基因;克隆;序列分析
摘  要:
提取毒害艾美耳球虫(En)配子体总RNA,应用RT-PCR进行gam22基因的克隆,测序后对其进行序列分析。结果表明,Engam22基因全长为713 bp,其中包含一个完整的开放阅读框,大小为561 bp,编码186个氨基酸,含有一个富含组氨酸和脯氨酸的特征性结构域;与柔嫩艾美耳球虫(Et)配子体Etgam22基因序列进行比对,同源性为97.7%。此配子体蛋白基因具有较高的保守性,可以作为抗球虫疫苗的候选基因。
译  名:
Cloning and sequence analysis of gam22 gene from Eimeria necatrix
作  者:
LIU Dandan;CAO Liqin;ZHU Yulan;XU Jinjun;WU Lili;JIN Wenjie;TAO Jianping;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,College of Veterinary Medicine,Yangzhou University;
关键词:
Eimeria necatrix;;gam22 gene;;cloning;;sequence analysis
摘  要:
Total RNA isolated from Eimeria necatrix gametocytes was utilized as template for RT- PCR amplification of gam22 gene,which encodes a gametocyte protein. Then the gene was sequenced. The results showed that the gam22 gene was 713 bp in length,which contained a complete open reading frame of 561 bp,encoding 186 amino acids. Its encoded protein contains a characteristic domain rich in histidine and proline. The gene had 97. 7% identity to that of gam22 of E. tenella,which presented a potential target for future recombinant subunit vaccines against coccidiosis.

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