当前位置: 首页 > 文章 > 抗病赤松组培丛生芽低温保存条件研究 南京林业大学学报(自然科学版) 2010,34 (5) 11-15
Position: Home > Articles > Study on cryopreservation of auxiliary buds of resistant Pinus densiflora Journal of Nanjing Forestry University(Natural Sciences Edition) 2010,34 (5) 11-15

抗病赤松组培丛生芽低温保存条件研究

作  者:
张红岩;吴小芹;朱丽华;谈家金
单  位:
南京林业大学森林资源与环境学院;江苏省有害生物入侵预防与控制重点实验室
关键词:
赤松;组织培养;丛生芽;低温保存;松材线虫病
摘  要:
从抗病赤松丛生芽的保存温度和保存时间以及山梨醇浓度、NAA和6-BA组合等方面探讨了离体条件下赤松组培丛生芽的低温保存条件。结果表明:保存温度为17℃时,丛生芽存活率达到96.7%;培养基中单独添加0.1mg/L的山梨醇或0.1mg/L多效唑均可缓解冷害,添加0.2mg/L的6-BA+(0.1~0.2)mg/L的NAA组合保存效果最好,可使恢复生长后丛生芽的增殖系数提高到5.9~6.0;不同基因型的丛生芽低温保存效果差异显著,其中,1#-A、7#-7、16#-2保存效果较好。
译  名:
Study on cryopreservation of auxiliary buds of resistant Pinus densiflora
作  者:
ZHANG Hong-yan,WU Xiao-qin*,ZHU Li-hua,TAN Jia-jin (College of Forest Resources and Environment,Nanjing Forestry University,Jiangsu Key Laboratory for Prevention and Management of Invasive Species,Nanjing 210037,China)
关键词:
Pinus densiflora;tissue culture;auxiliary buds;cryopreservation;pine wood nematode disease
摘  要:
The conditions for cryopreservation were studied at storage temperature,storage time,sorbitol contention and the combination of 6-BA and NAA levels for better understanding of reservation and effective utilization of resistant Pinus densiflora.The results showed that,when reserved at 17 ℃,survival rate of auxiliary buds could be up to 96.7 %,sorbitol and paclobutrazol could alleviate chilling injury,0.2 mg/L 6-BA + (0.1—0.2) mg/L NAA was the best combination of cryopreservation,which could improve the multiplication coefficient to 5.9—6.0 in the course of recovery growth,and the effects of cryopreservation were greatly different from each other,1#-A,7#-7,16#-2 were elite genotype.It also provided reference for the reservation of other germplasm resources.

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