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Position: Home > Articles > Effects of Different Factors on Chelating Activity of Casein Enzymatic Hydrolysate with Ferrous Salt FOOD SCIENCE 2008,29 (5) 145-148

不同因素对酪蛋白酶解产物螯合亚铁盐能力的影响

作  者:
毛学英;李明;李敏;徐世平;许成才;谷俊男;任发政;刘坤晓
单  位:
中国农业大学食品科学与营养工程学院
关键词:
酪蛋白;酶解产物;亚铁;螯合
摘  要:
本研究以干酪素酪蛋白为原料,对影响酪蛋白酶解产物螯合Fe2+反应的因素进行了初步研究,为获得高效生物补铁剂以预防缺铁性贫血提供理论依据。研究表明,FeSO4与酪蛋白酶解产物的螯合能力显著高于(NH4)2Fe(SO4)2或FeCl2与酪蛋白酶解产物的螯合能力(p<0.05);不同酶解时间获得的酪蛋白酶解产物与FeSO4的螯合能力不同,7h时获得的酶解产物螯合FeSO4的能力最强;当酪蛋白酶解产物与FeSO4共同作用10min、硫酸亚铁与酪蛋白酶解产物的质量比为1:2、反应体系pH为6.0,反应温度为40℃时螯合效果较好。
译  名:
Effects of Different Factors on Chelating Activity of Casein Enzymatic Hydrolysate with Ferrous Salt
作  者:
MAO Xue-ying1,LI Ming1,LI Min1,XU Shi-ping1,XU Cheng-cai1,GU Jun-nan1,REN Fa-zheng1,LIU Kun-xiao2(1.College of Food Science and Nutritional Engineering,China Agricultural University,Key Laboratory of Functional Dairy,Ministry of Education and Beijing City,Beijing 100083,China;2.Affiliated Middle School of Northern Jiaotong University,Beijing 100081,China)
关键词:
casein;enzymatic hydrolysate;ferrous;chelate
摘  要:
This study explored the effects of the kinds of ferrous salts,hydrolysis time,co-incubation time,mass ratio,reaction pH and temperature on the chelating activity of casein hydrolysate with ferrous salt,in order to prepare some basic data for preparation of a product rich in bio-iron.Peptides produced by enzyme hydrolysis of casein reacted with Fe2+.Results showed that the chelating activity of FeSO4 with casein hydrolysate was significantly higher than that of(NH4)2Fe(SO4)2 and FeCl2(p <0.05).The chelating activity of casein hydrolysate obtained at different hydrolysis time differed,and when hydrolysis time 7 h was the best.When the co-incubation time of casein hydrolysate and FeSO4,mass ratio,reaction pH and temperature were 10 min,1:2,6.0 and 40 ℃ respectively,the chelating effect was optimum.

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