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当前位置: 首页 > 文章 > 杭白菊挥发油成分分析及β-榄香烯含量的测定 食品科学 2008,29 (9) 506-510
Position: Home > Articles > Composition Analysis of Volatile Oil Extracted from Chrysanthemum moriflium Ramat and Determination of β-Elemene FOOD SCIENCE 2008,29 (9) 506-510

杭白菊挥发油成分分析及β-榄香烯含量的测定

作  者：
孙桂菊;张菲菲;马永建;杨立刚;康学军;王少康
单  位：
江苏省疾病预防控制中心;复旦大学医学院附属妇产科医院;东南大学公共卫生学院营养与食品卫生系
关键词：
杭白菊;挥发油;分析;β-榄香烯
摘  要：
目的:分析杭白菊挥发油的化学成分并建立杭白菊中β-榄香烯的测定方法。方法:利用气相色谱-质谱联用仪分析杭白菊挥发油各组成成分,并使用气相色谱内标法测定杭白菊中β-榄香烯的含量。结果:杭白菊挥发油经气相色谱-质谱联用仪分析,共分离出125个峰,鉴定出59种化合物,占挥发油总量的61.74%;经分析,β-榄香烯线性范围为0.006～0.096mg/ml,RSD为0.85%,平均回收率为95.23%,在杭白菊中含量为12.98mg/kg。结论:利用气-质联用分析技术鉴定出菊花挥发油中的59种化合物,主要成分为单萜烯类、倍半萜烯类及其含氧衍生物等,杭白菊中具有抗肿瘤活性物质β-榄香烯的含量为12.98mg/kg。
译  名：
Composition Analysis of Volatile Oil Extracted from Chrysanthemum moriflium Ramat and Determination of β-Elemene
作  者：
SUN Gui-ju1,ZHANG Fei-fei2,MA Yong-jian3,YANG Li-gang1,KANG Xue-jun1,WANG Shao-kang1 (1. Department of Nutrition and Food Hygiene,School of Public Health,Southeast University,Nanjing 210009,China; 2. Obstetrics and Gynecology Hospital,Medical Center of Fudan University,Shanghai 200011,China; 3. Jiangsu Provincial Center for Disease Prevention and Control,Nanjing 210009,China)
关键词：
Chrysanthemum moritilum Ramat;volatile oil;analysis; β-elemene
摘  要：
Objective: To analyze the ingredients in volatile oil extracted from Chrysanthemum moriflium Ramat and to determine the content of β-elemene in the volatile oil. Method: GC- MS analysis was used to analyze the composition of the volatile oil and the ninner standard method was used to determine the content of β-elemene . Results : 59 compounds among 125 peaks separated out by GC-MS were indentified , accounting for 61.74% of total quantity of the volatile oil. The linear range of β- elemene is from 0.006 mg/ml to 0.096 mg/ml. The relative standard derivation( RSD) of repeated experiment is 0.85 % . The average recovery is 95.23 %. And the content of β-elemene in chrysanthemum moriflium Ramat is 12.98 mg/kg. Conclusions: The analysis of main composition of volatile oil from Chrysanthemum moriflium Ramat by GC- MS can enrich the study on constituents of this plant in the world and lay the foundation for further utilization. The internal standard method for determine the content of β-elemene in the volatile oil is simple, accurate, reproducible and practical. This study can promote development and utilization of traditional Chinese herbal medicines.

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