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当前位置: 首页 > 文章 > 内生真菌印度梨形孢对水稻苗期耐盐性的影响 南方农业学报 2019,50 (4) 719-725
Position: Home > Articles > Effects of endophytic fungus Piriformospora indica on salt stress tolerance of rice seedling Journal of Southern Agriculture 2019,50 (4) 719-725

内生真菌印度梨形孢对水稻苗期耐盐性的影响

作  者：
刘雪琳;朱志炎;何勇;叶开温;田志宏
单  位：
长江大学生命科学学院
关键词：
印度梨形孢;水稻;耐盐性;酶活性;基因表达
摘  要：
[目的]研究印度梨形孢(Piriformospora indica)对盐(NaCl)胁迫后水稻幼苗生长发育的影响,明确印度梨形孢提高水稻幼苗耐盐性的机理.[方法]采用不同浓度(0、50、100、150和200 mmol/L)的盐(NaCl)溶液处理水稻幼苗,72 h后加入5×105个/mL的印度梨形孢孢子悬浮液10 mL侵染水稻根部(接种处理),以未侵染为对照(不接种,CK);20 d后测定水稻幼苗叶片的丙二醛(MDA)、脯氨酸(Pro)、叶绿素、可溶性糖和可溶性蛋白含量及抗氧化酶活性等生理指标,并利用实时荧光定量PCR(qPCR)检测耐盐相关基因的表达情况.[结果]盐胁迫处理后,与CK相比,接种处理水稻幼苗的MDA含量降低,叶绿素、可溶性糖和可溶性蛋白含量及抗氧化酶活性升高.其中,盐胁迫浓度为150和200 mmol/L时,CK的MDA含量是接种处理的1.5和2.8倍;盐胁迫浓度为150 mmol/L时,接种处理的Pro含量是CK的2.1倍;盐胁迫浓度为200 mmol/L时,接种处理的可溶性糖含量是CK的1.8倍;差异均达显著水平(P<0.05,下同).qPCR检测结果表明,经盐胁迫后接种处理水稻幼苗叶片中耐盐相关基因SNAC1、OsNAC6、OsBZIP23和OsDREB2A的表达量均高于CK,各基因表达量均在100 mmol/L处理下达最大值,其中SNAC1和OsNAC6基因表达量分别是CK的1.5和1.1倍,差异达显著水平.[结论]接种印度梨形孢可降低盐胁迫后水稻幼苗叶片的MDA含量,提高抗氧化酶活性及Pro、叶绿素、可溶性糖和可溶性蛋白含量,同时促使水稻叶片中耐盐胁迫相关基因的表达量上调,从而增强水稻幼苗对盐胁迫的耐受性.印度梨形孢作为生物菌肥在农业生产中有潜在的利用价值.
译  名：
Effects of endophytic fungus Piriformospora indica on salt stress tolerance of rice seedling
作  者：
LIU Xue-lin;ZHU Zhi-yan;HE Yong;YE Kai-wen;TIAN Zhi-hong;College of Life Science,Yangtze University;
关键词：
Piriformospora indica;;rice;;salt tolerance;;enzyme activity;;gene expression
摘  要：
【Objective】Study on the effects of Piriformospora indica on the growth and development of rice seedlings under salt stress(NaCl)was conducted,and the mechanism of P. indica improving salt tolerance of rice seedlings was also researched.【Method】Rice seedlings were treated with different concentrations(0,50,100,150 and 200 mmol/L)of salt(NaCl)solution. After 72 h,5×10~5 spore/mL P. indica spore suspension was added to infect rice roots(inoculation treatment). With the absence of dip-dyeing as control(CK),20 d later,the physiological indicators such as malondialdehyde(MDA),proline(Pro),chlorophyll,soluble sugar,soluble protein contents and antioxidant enzyme activities in rice seedling leaves were determined. Real-time fluorescence quantitative PCR(qPCR)was used to detect the expression of salt-tolerant genes.【Result】After salt stress treatment,compared with CK,MDA content decreased,chlorophyll,soluble sugar and soluble protein content and antioxidant enzyme activities increased in infected rice seedlings. Among them,when the salt concentrations were 150 and 200 mmol/L,the MDA contents of CK were as 1.5 and 2.8 times as that of P. indica treatment;when the salt concentration was 150 mmol/L,the Pro content of P. indica inoculated rice was as 2.1 times as that of CK;when the salt concentration was 200 mmol/L,the soluble sugar content of P. indica treatment was as 1.8 times as that of CK;the differences were significant(P<0.05,the same below). The results of qPCR showed that the expression levels of salt-tolerant genes SNAC1,OsNAC6,OsBZIP23 and OsDREB2 A in the leaves of rice seedlings after salt stress were higher than those of CK,and the expression level of each gene reached the maximum at 100 mmol/L.The expression levels of SNAC1 and OsNAC6 genes in P. indica seedlings were as 1.5 and 1.1 times as those of CK,respectively,and the differences were significant.【Conclusion】Inoculation with P. indica can decrease the MDA content and increase the activities of antioxidant enzymes,Pro,chlorophyll,soluble sugar and soluble protein contents in rice seedling leaves under salt stress,and also promote the up-regulation of salt tolerance-related genes in rice leaves,and finally enhance the tolerance of rice seedlings to salt stress. P. indica has potential to be uses as a biological fertilizer in agricultural production.

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