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Position: Home > Articles > Study on the Antioxidant Activity of Sepia Ink Peptides in vitro Journal of Anhui Agricultural Sciences 2013 (27) 10971-10973+11025

乌贼墨多肽体外抗氧化活性研究

作  者:
景奕文;杨最素;丁国芳;黄芳芳
单  位:
浙江省海洋生物医用制品重点工程技术研究中心;浙江海洋学院食品与医药学院
关键词:
乌贼墨;多肽;抗氧化
摘  要:
[目的]研究乌贼墨多肽体外抗氧化活性。[方法]采用分光光度法测定多肽对2,2’-连氮基-双-(3-乙基苯并二氢噻唑林-6-磺酸)二铵盐(ABTS)、1,1-二苯基-2-三硝基苯肼(DPPH)、羟自由基(·OH)的清除能力和还原能力,利用琼脂糖凝胶电泳检测多肽对羟自由基诱导的DNA损伤的保护作用。[结果]乌贼墨多肽具有明显清除ABTS自由基(半数有效浓度EC50为3.45 mg/ml)、·DPPH自由基(EC50为4.34 mg/ml)和羟自由基(EC50为3.30 mg/ml)的能力;当乌贼墨多肽的浓度达到5.00 mg/ml时,还原力达到0.387。[结论]乌贼墨多肽具有较好的抗氧化活性。
译  名:
Study on the Antioxidant Activity of Sepia Ink Peptides in vitro
作  者:
JING Yi-wen;Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products,School of Food Science and Pharmacy of Zhejiang Ocean University;
关键词:
Sepia ink;;Polypeptide;;Antioxidation
摘  要:
[Objective]To study the antioxidant activity of sepia ink peptides in vitro. [Method]The spectrophotometry method was adopted to determine the scavenging and reducing capacity of polypeptide on ABTS,1,1-diphenyl-2-picrylhydrazyl (·DPPH) and hydroxyl radical; the protective effect of the peptides against hydroxyl-radical-induced DNA damage was measured by DNA agarose electrophoresis. [Result] The results showed that the ABTS,DPPH and hydroxyl free radicals radical-scavenging activity were increased with concentrations of the peptides,and the EC50values were 3. 45 mg/ml,4. 34 mg/ml,3. 30 mg/ml,respectively. [Conclusion] The peptides isolated from sepia ink hydrolyzate exhibited an obvious capability of antioxidation.

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