单 位：
华中农业大学食品科学技术学院,环境食品学教育部重点实验室;华中农业大学食品科学技术学院
关键词：
高粱原花青素;黏附;荧光标记;双向凝胶电泳;基质辅助激光解吸电离飞行时间质谱
摘 要：
以高纯度高粱原花青素(sorghum procyanidins,SPC)混合物和高粱原花青素四聚体(sorghum procyanidins tetramers,SPC-Ⅲ)为原料,采用荧光标记的方法比较其抑制Streptococcus mutans Ingbritt(c)黏附效果,在此基础上结合双向凝胶电泳(two-dimensional electrophoresis,2-DE)以及基质辅助激光解吸电离飞行时间质谱(matrix-associated laser dissociation/ionization time of fl ight mass spectrometry,MALDI-TOF-MS)探究其干预S.mutans Ingbritt(c)黏附的主要途径并期望阐明其作用机理。结果表明:SPC-Ⅲ比SPC混合物抑制S.mutans Ingbritt(c)体外黏附的效果更强,SPC-Ⅲ处理细菌的抑制效果强于处理获得性膜的抑制效果,是其抑制细菌黏附主要途径;类葡萄糖基转移酶(glycosyltransferases,GTFs)活性蛋白(3.10-Ⅱ)经SPC-Ⅲ作用前后的2-DE图有明显差异,差异点均为S.mutans中的不同蛋白酶类,其中A24可能是一种新蛋白。
译 名：
in vitro Adhesion Pathways and Mechanisms of Streptococcus mutans Ingbritt(c) during Intervention with Proanthocyanidins Tetramers
作 者：
HUANG Man;CAI Xin;YU Jia-nan;TANG Cui-e;LIU Rui;Key Laboratory of Environment Correlative Dietology,Ministry of Education,College of Food Science and Technology,Huazhong Agricultural University;
关键词：
sorghum proanthocyanidins;;adhesion;;fluorescence labeling;;two-dimensional electrophoresis;;matrix-associated laser dissociation/ionization time of f light mass spectrometry
摘 要：
In this study, high purity proanthocyanidins(SPC) and proanthocyanidins tetramers(SPC-III) from sorghum episperm were compared for their inhibitory effects on the adhesion of Streptococcus mutans Ingbritt(c) through fluorescence labeling. By combining two-dimensional electrophoresis(2-DE) and matrix-associated laser dissociation/ ionization time of flight mass spectrometry(MALDI-TOF-MS), the major intervening pathways were investigated, and the underlying mechanisms were explored. The results showed that SPC-III(tetramers) had better inhibitory effect than SPC and had stronger inhibitory effect than SPC-III-treated salivary acquired pellicle on the adhesion of S. mutans Ingbritt(c), which was the major pathway to inhibit bacterial adhesion. Two-dimensional electrophoretograms of 3.10-II treated by SPC-III or not showed significant differences. All different points were different proteases derived from S. mutans, in which A24 was suggested to be a new protein that has never been detected in S. mutans previously.
