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Position: Home > Articles > Cloning and Sequencing of the cDNA of Neuropeptide Y Receptor Y1 from Pig Journal of Anhui Agricultural Sciences 2008 (36) 15797-15798

猪NPY受体Y1 cDNA的克隆及分析

作  者:
周春宝;倪黎纲;丁家桐;包文斌
单  位:
扬州大学动物科学与技术学院;江苏畜牧兽医职业技术学院
关键词:
苏姜猪;NPY-Y1基因;RT-PCR扩增;序列分析
摘  要:
[目的]研究猪NPY-Y1受体在猪激素分泌及发育生理功能中的调节作用。[方法]从初情期的苏姜猪母猪中提取下丘脑组织总RNA,RT-PCR扩增出NPY-Y1 cDNA,扩增产物克隆入pGEM-T easy载体中进行序列测定。[结果]试验扩增产物与GeneBank公布的猪NPY-Y1序列比较,其同源性达到100%。[结论]为下一步研究猪NPY-Y1基因的生理功能和作用机理奠定了基础。
译  名:
Cloning and Sequencing of the cDNA of Neuropeptide Y Receptor Y1 from Pig
作  者:
ZHOU Chun-bao et al (Jiangsu Animal Husbandry and Veterinary College,Taizhou,Jiangsu 225300)
关键词:
Su-jiang pig;NPY-Y1 gene;RT-PCR amplification;Sequencing
摘  要:
[Objective]The research aimed to study the regulating function of neuropeptide Y receptor Y1on the hormone secretion and development physiology in pig.[Method] Pig NPY-Y1 gene was obtained using reverse transcription polymerase chain reaction amplification(RT-PCR) of total RNA extracted from hypothalamus tissue of pituitary in Su-jiang pig.The RT-PCR products was subcloned into pGEM-T easy vector and the correctness of the sequences was confirmed by sequence analysis.[Result]The result of sequence analysis showed that its homology with NPYY1 cDNA from GenBank was up to 100%.[Conclusion] These data indicated that the NPY-Y1 cDNA was cloned correctly,which would play a great role in the research of its physiological functions and mechanism.

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