中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 豆状囊尾蚴外泌体的分离鉴定及其对家兔免疫应答的调节作用 中国兽医科学 2020 (3) 307-313
Position: Home > Articles > Isolation and characterization of exosomes derived from Cysticercus pisiformis and its immunomodulation on rabbits' immune response Chinese Veterinary Science 2020 (3) 307-313

豆状囊尾蚴外泌体的分离鉴定及其对家兔免疫应答的调节作用

作  者：
王立群;李艳萍;张少华;梁盼红;李涛善;刘婷丽;骆学农
单  位：
甘肃农业大学动物医学院;中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室;扬州大学江苏省动物重要疫病与人兽共患病防控协同创新中心
关键词：
豆状囊尾蚴;外泌体;免疫调节;细胞因子;减虫率
摘  要：
为探究豆状囊尾蚴外泌体对家兔免疫应答的调节作用,以收集的豆状囊尾蚴分泌排泄产物(ESP)为材料,通过差速离心法分离外泌体,经透射电镜和Western-blot分别对其形态和标志分子进行鉴定。分别用外泌体和ESP免疫家兔,ELISA方法检测家兔血清中特异性抗体和血清细胞因子水平,并通过人工攻虫感染评价其免疫保护效果。结果显示,豆状囊尾蚴外泌体直径为30～150 nm,呈圆形或椭圆形,有完整的脂质双分子层膜结构,且含有外泌体标志蛋白CD63。而且,外泌体免疫组血清特异性抗体、IL-10和IL-4的水平显著升高(P<0.05),而IFN-γ和TNF-α显著降低(P<0.05)或差异不显著。经剖检发现,豆状囊尾蚴ESP免疫组和外泌体免疫组的减虫率分别为65.17%和24.88%。结果表明,外泌体通过上调宿主抑炎因子的表达,诱导宿主产生了Th2型细胞免疫应答,从而有利于囊尾蚴在宿主体内的存活。
译  名：
Isolation and characterization of exosomes derived from Cysticercus pisiformis and its immunomodulation on rabbits' immune response
作  者：
WANG Li-qun;LI Yan-ping;ZHANG Shao-hua;LIANG Pan-hong;LI Tao-shan;LIU Ting-li;LUO Xue-nong;State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;College of Veterinary Medicine,Gansu Agricultural University;Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses/Yangzhou University;
关键词：
Cysticercus pisiformis;;exosomes;;immunoregulation;;cytokine;;worm reduction rate
摘  要：
To explore the regulatory role of Cysticercus pisiformis exosomes in immune response to rabbits,the exosomes from excretory-secretory products(ESP) of C.pisiformis were isolated by differential centrifugation.The morphology of exosomes was observed by transmission electron microscopy(TEM) and the marker protein CD63 was analyzed by Western-blot.Subsequently,rabbits were immunized with exosomes and ESP respectively,and then the level of specific antibodies and cytokines were detected by ELISA.Moreover,the immunoprotective effect was evaluated after artificial challenge infection with T.pisiformis.The results showed that the C.pisiformis exosomes were 30 to 150 nm in diameter with a complete lipid bilayer membrane structure,which was spherical or oval,and reacted with anti-CD63 antibodies.The levels of specific antibodies,IL-10 and IL-4 were significantly increased in exosomes immunized rabbits(P <0.05),while the level of IFN-γ was significantly decreased(P <0.05)and TNF-α performed no difference.The worm reduction rates for ESP group and exosomes group were 65.17% and 24.88% after necropsy,respectively.These results implied that exosomes of C.pisiformis could induce the Th2 type cellular immune response by promoting the host anti-inflammatory cytokines expression,which would contribute to the survival of Cysticercus in rabbits.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46