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当前位置: 首页 > 文章 > 交联壳聚糖磁性微球的制备及固定化果胶酶研究 中国农学通报 2012,28 (33) 204-208
Position: Home > Articles > The Preparation of Magnetic Microsphere of Cross-linked Chitosan and Immobilization of Pectinase Chinese Agricultural Science Bulletin 2012,28 (33) 204-208

交联壳聚糖磁性微球的制备及固定化果胶酶研究

作  者：
林建城;陈燕红;张雄
单  位：
莆田学院环境与生命科学系
关键词：
磁性微球;交联壳聚糖;果胶酶;固定化;酶学性质
摘  要：
以交联壳聚糖磁性微球为载体制备固定化果胶酶,并研究固定化酶的酶学性质与操作稳定性。通过Fe3O4磁核与壳聚糖制备磁性微球载体,戊二醛交联后对果胶酶进行固定,利用正交试验确定固定化酶制备条件,比较研究了固定化酶与游离酶的酶学性质。结果表明:在Fe2+:Fe3+体积比为1:1的溶液中,制得Fe3O4磁核;1.0gFe3O4磁核,3.0%壳聚糖,在3.0%戊二醛中交联4h,3.0mg/mL果胶酶在pH4.0下固定化反应1h,制备的固定化酶酶活力的回收率高达68.4%。对固定化酶酶学特性研究表明:最适pH4.0,最适温度50℃,酸碱稳定性范围为pH2.6～5.6,温度稳定性得到提高,70℃下剩余酶活力为82.8%,米氏常数Kmapp值为1.38mg/mL,固定化酶连续使用6次还保留61.0%酶活力。说明以交联壳聚糖磁性微球为载体制备的固定化果胶酶,机械强度大、弹性好,酶活力回收率较高,操作稳定性好。
译  名：
The Preparation of Magnetic Microsphere of Cross-linked Chitosan and Immobilization of Pectinase
作  者：
Lin Jiancheng,Chen Yanhong,Zhang Xiong(Department of Environment & Life Sciences,Putian University,Putian Fujian 351100)
关键词：
magnetic microsphere;cross-linked chitosan;pectinase;immobilization;properties of enzymology
摘  要：
Pectinase was immobilized with magnetic microsphere of chitosan and crosslinked with glutaraldehyde.The partial properties of immobilized enzyme were investigated.The magnetic microspheres were prepared with Fe3O4 magnetic nucleus and chitosan.Pectinase was immobilized while microspheres were crosslinked with glutaraldehyde.The preparation conditions of immobilized enzyme were determined by orthogonal test design.The partial properties of immobilized enzyme and free enzyme were compared and studied.The results showed that,Fe3O4 magnetic nucleus could be made out when Fe 2+:Fe 3+ was 1:1(volume ratio).When the amount of Fe3O4 was 1.0 g,chitosan concentration of 3.0%,3.0 mg/mL pectinase amount,treated with 3.0% glutaraldehyde for 4 h,and the process time was 1 h under pH 4.0,the recovery rate of magnetic immobilized pectinase activity could reach to 68.4%.The optimum pH and optimum temperature of immobilized pectinase for the hydrolysis of pectin were determined to be pH 4.0 and 50℃,respectively.The immobilized pectinase was stable in the pH ranges from 2.6 to 5.6,and had good thermal stability under 70℃,which the residual enzyme activity was also 82.8% after treated at 70℃ for one hour.The apparent Michaelis constant of immobilized pectinase K m app equaled to 1.38 mg/mL.The recovery activity of immobilized enzyme was more than 61.0% after repeated six times.The immobilized pectinase prepared with magnetic microsphere of chitosan were good enough for the strength,the flexibility and the recovery rate,and had good operation stability.

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