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Position: Home > Articles > Structure and Function of Bma-COP Gene in Silkworm( Bombyx mori. ) Southwest China Journal of Agricultural Sciences 2015,28 (4) 1840-1846

家蚕Bmβ-COP基因结构及功能

作  者:
杜周和;樊玉杰;刘俊凤;刘彬斌;濮佳;陈义安
单  位:
中国科学院上海生命科学研究院植物生理生态研究所;四川省农业科学院蚕业研究所
关键词:
家蚕;Bmβ-COP基因;膜泡运输;选择性剪接;丝蛋白
摘  要:
采用克隆测序、生物信息学分析及表达谱检测等方法,分析了家蚕Bmβ-COP基因的结构和功能特征,探索其与丝蛋白合成的关系。结果表明,Bmβ-COP基因在家蚕基因组中为单一拷贝,也没有选择性剪接异构体存在;在不同产丝能力的家蚕品种及其不同组织间没有表达差异;Bmβ-COP基因与不同家蚕品种间丝物质生产能力差异没有直接关联,但破坏它会影响丝腺的发育和功能,对家蚕丝蛋白合成、分泌产生不利影响。
译  名:
Structure and Function of Bma-COP Gene in Silkworm( Bombyx mori. )
作  者:
DU Zhou-he;FAN Yu-jie;LIU Jun-feng;LIU Bin-bin;PU Jia;CHEN Yi-an;Sericultural Research Institute,Sichuan Academy of Agricultural Sciences;Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,CAS;
关键词:
Bombyx mori;;Bmβ-COP gene;;Vesicular transport;;Alternative splicing;;Silk protein
摘  要:
By cloning,sequencing and bioinformatics analysis,the Bmβ-COP gene was identified. The expression pattern in different tissues was detected to reveal the relationship between Bmβ-COP expression and silk productivity in silkworm. The result showed that in genome of B. mori,β-COP is the unique locus encoding β-COP protein. No alternative splicing event occurres. There is no difference among the expression patterns of Bmβ-COP gene in different tissues of different silkworm variety. No direct relationship between Bmβ-COP expression and different silk protein production of silkworm varieties was identified,but knocking down Bmβ-COP perturbs the development and function of silk gland,resulting in worse silk productivity.

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