作 者：
王振焕;陈雄英;胡清华;钟华;邓峰美;何芳
单 位：
石河子大学医学院/新疆地方与民族高发病重点实验室;华中科技大学同济医学院/卫生部呼吸系疾病重点实验室
关键词：
细胞外钙受体;小凹蛋白-1;脐静脉内皮细胞
摘 要：
为探讨人脐静脉内皮细胞(HUVEC)中小凹蛋白-1与细胞外钙受体表达及定位,为进一步功能研究提供理论依据,采用Western-blot和Rt-PCR及免疫荧光技术检测HUVEC中小凹蛋白-1与细胞外钙受体mRNA和蛋白表达及定位。结果显示:(1)形态学观察和免疫细胞化学法测Ⅷ因子抗原,95%以上的细胞呈阳性着色,证实细胞为HUVEC。(2)RT-PCR检测到HUVEC中459bp和260bpmRNA表达,Western-blot测到HUVEC中22KD和150-160KD蛋白表达。(3)免疫荧光检测到小凹蛋白-1与细胞外钙受体定位于人脐静脉内皮细胞膜。由此可知,人的HUVEC中有小凹蛋白-1与细胞外钙受体表达,两者是否共定位及其功能有待进一步研究。
译 名：
Expressions of Extracellular Calcium-sensing Receptor and Caveolin-1 in Human Umbilical Vein Endothelial Cells
作 者：
WANG Zhenhuan1,CHEN Xiongying1,HU Qinghua2,ZHONG Hua1,DENG Fengmei1,HE Fang1(1 Medical College of Shihezi University/Ministry of Education Key Laboratory of Xinjiang Endemic and Ethnic Diseases,Shihezi 832002,China;2 Tongji Medical College,Huazhong University of Science and Technology/Key Laboratory of Pulmonary Disease of Ministry of Health of China,Wuhan 430030,China)
关键词：
extracellular calcium receptor;cavelin-1;human umbilical vein endothelial cells
摘 要：
To investigate the expression of caveolin-1 and extracellular calcium receptor(CaR) and localization in human umbilical vein endothelial cells(HUVECs),which provide a theoretical basis for further co-localization and functional studies.Methods:The expressions of caveolin-1 and CaR mRNA and protein in HUVECs were detected by Western-blot and Rt-PCR,and their localizations were investigated by immunofluorescence.Results:(1) We identified HUVECs in which over 95% of cells were positive staining by morphology and immunocytochemistry measuring Ⅷ factor antigen.(2) The expression of 459bp,260bpmRNA and 22KD,150-160KD protein were detected in HUVEC by Western-blot detected and RT-PCR,respectively.(3)Caveolin-1 and CaR were localization in HUVEC membrane by immunofluorescence.Conclusions:Our observations demonstrated that there were caveolin-1 and CaR in HUVECs,and they were localization in the cell membrane.Whether they were co-localization and the relation between the expression and its function should be further studied.
