当前位置: 首页 > 文章 > 细胞外钙受体调节人脐静脉内皮细胞游离钙离子浓度的研究 石河子大学学报(自然科学版) 2010,28 (4) 63-66
Position: Home > Articles > Extracellular Ca-sensing Receptor Regulating Intracellular Ca~(2+) Concentration in Human Umbilical Vein Endothelial Cells Journal of Shihezi University(Natural Science) 2010,28 (4) 63-66

细胞外钙受体调节人脐静脉内皮细胞游离钙离子浓度的研究

作  者:
陈雄英;王振焕;胡清华;钟华;邓峰美;何芳
单  位:
石河子大学医学院/新疆地方与民族高发病重点实验室;华中科技大学同济医学院/卫生部呼吸系统疾病重点实验室
关键词:
细胞外钙受体;Calhex231;细胞内钙离子浓度
摘  要:
为探讨人脐静脉内皮细胞(HUVEC)游离钙离子浓度是否受细胞外钙受体(CaR)的调节。采用Westernblot及RT-PCR技术检测CaR在HUVEC中蛋白及mRNA表达。使用CaR激动剂精胺,负性变构调节剂Calhex231,通过钙荧光探针(Fura-2/AM)负载HUVEC检测细胞内钙离子浓度([Ca2+]i)。结果显示:(1)HUVEC中有CaR的表达。(2)在Fura-2/AM负载HUVEC中加入精胺刺激,[Ca2+]i为△ratio=0.24±0.01,先加入Calhex231作用1min后再加入Calhex231+精胺刺激,[Ca2+]i为△ratio=0,两组比较差异有统计学意义(P<0.05)。由此可知,CaR调节HUVEC游离钙离子浓度。
译  名:
Extracellular Ca-sensing Receptor Regulating Intracellular Ca~(2+) Concentration in Human Umbilical Vein Endothelial Cells
作  者:
CHEN Xiongying1,WANG Zhenghuang1,HU Qinghua2,ZNONG Hua1,DENG Fengmei1,HE Fang1 (1 Medical College,Shihezi University/Ministry of Education Key Laboratory of Xinjiang Endemic and Ethnic Diseases,Shihezi 832002,China;2 Tongji Medical College,Huazhong University of Science and Technology/Key Laboratory of Pulmonary Disease of Ministry of Health of China,Wuhan 430030,China)
关键词:
calcium-sensing receptor;Calhex231;intracellular Ca2+ concentration
摘  要:
To study whether intracellular Ca2+concentration ([Ca2+]i) is regulated by Ca-sensing receptor (CaR) in human umbilical vein endothelial cells(HUVEC).CaR protein was demonstrated by Western blot.The mRNA expression of CaR was investigated by RT-PCR in HUVEC.The fluorescence Ca2+ indicator Fura-2 /AM was used to observe [Ca2+]i in the presence of CaR agonists spermine and negative allosteric modulator Calhex231.(1) CaR is present in HUVEC.(2)There was significantly difference in [Ca2+]i between spermine (ratio=0.24 ±0.01) and Calhex231+spermine (ratio=0) (P<0.05).CaR regulates [Ca2+]i in HUVEC.

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