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当前位置: 首页 > 文章 > γ-射线对粳稻幼穗培养及再生植株后代变异的影响 上海农业学报 1992 (2) 19-23
Position: Home > Articles > EFFECTS OF γ-RAYS ON YOUNG PANICLE CULTURE AND THE PROGENY VARIATION OF REGENERATED PLANTS IN JAPONICA RICE Acta Agriculturae Shanghai 1992 (2) 19-23

γ-射线对粳稻幼穗培养及再生植株后代变异的影响

作  者：
张建军
单  位：
上海市农业科学院作物育种栽培研究所
关键词：
粳稻;离体辐照;γ-射线;辐照敏感性;变异
摘  要：
本实验以6种粳稻为试材,研究了γ-射线对离体培养及再生植株后代变异的影响。结果表明,辐照对离体培养的作用随基因型、辐照组织、剂量和剂量率而异,但以负效应为主。不同组织对辐照的敏感性依次为:幼穗>分化愈伤组织>脱分化愈伤组织。辐照幼穗的合适剂量为19Gy以下,而辐照愈伤组织的适宜剂量为19—47.5Gy。再生植株变异率的高低与辐照剂量和辐照组织对射线的敏感性密切相关。综合考虑辐照对离体培养及再生植株后代变异的影响,以低剂量(≤19Gy)辐照幼穗的方法较为合适。
译  名：
EFFECTS OF γ-RAYS ON YOUNG PANICLE CULTURE AND THE PROGENY VARIATION OF REGENERATED PLANTS IN JAPONICA RICE
作  者：
Zhang Jianjun ( Crop Breedinq and Cultivation Research Institute, shanghai Academy of Agricultural Sciences , Shanghai 201106)
关键词：
Japonica rice; In vitro radiation; γ-rays; Radiosensitivity; Variation
摘  要：
The effect of γ-rays on tissue culture and variation of regenerated plants were studied with 6 Japonica rice varieties. The results showed that the action of radiation on tissue culture was different with genotypes, tissues irradiated, dosages and dosage rates; and γ-rays usually had negative action on tissue culture. The sequence of radiosensitivity of the tissues irradiated was young panicle> differentiating callus>dedifferentiating callus. The dosages under 19Gy seem to be appropriate for irradiating young panicle,whereas the dosages from 19 to 47.5 Gy would be appropriate for irradiating callus. The variation of regenerated plants was mainly affected by irradiating dosage and tissue irradiated. It is an effective method to irradiate young panicle with γ-rays at low dosage (≤19 Gy).

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