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当前位置: 首页 > 文章 > 大兴安岭野生蓝莓离体快繁关键技术 林业科技 2013,38 (4) 5-7
Position: Home > Articles > Key Technology of Rapid Propagation in Vitro of Daxing'anling Wild Blueberry Forestry Science & Technology 2013,38 (4) 5-7

大兴安岭野生蓝莓离体快繁关键技术

作  者：
李京;张妍妍;田新华
单  位：
黑龙江省林业科学研究所速生林木培育重点实验室
关键词：
野生蓝莓;离体快繁;关键技术;优化筛选
摘  要：
利用大兴安岭野生蓝莓优良种源的茎段为外植体,对野生蓝莓离体培养所需的最佳灭菌方法、最适的增殖和生根培养基及培养条件进行研究的结果表明:野生蓝莓离体快繁最佳的灭菌方法为0.1%升汞浸泡3min;最适增殖培养基为WPM(改良)+ZT 0.5mg/L,分化率可高达90%左右;最适生根培养基为1/2WPM(改良)+IBA 1mg/L,接种后首先放置于暗培养室中暗培养20d,然后转移到光照下培养,生根率可高达65%以上。
译  名：
Key Technology of Rapid Propagation in Vitro of Daxing'anling Wild Blueberry
作  者：
LI Jing(Key Laboratory of Fast-growing Tree Cultivating,Forestry Research Institute of Heilongjiang Province,Harbin 150081)
关键词：
Wild blueberry;Rapid propagation in vitro;Key technology;Optimization screening
摘  要：
The stem segments of wild blueberry were used as the explants to study the best sterilizing method of wild blueberry in vitro culture,the optimal proliferation and rooting culture medium,and culture condition.The results showed that the best sterilizing method of wild blueberry in witro rapid propagation was 0.1% mercuric chloride soaked for 3min;the optim um culture medium was using WPM(improved) + ZT 0.5mg/L that the differentiation rate can be as high as 90%;the optimal rooting medium was 1 /2 WPM(improved) + IBA 1mg / L.The inoculated stem segments cultured in darkness for 20d,and then cultivated wild blueberry seedling in the light.The rooting rate can be as high as 65%.

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