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当前位置: 首页 > 文章 > 基质固相分散-高效液相色谱法测定川味香肠中的苏丹红染料 食品工业科技 2012,33 (9) 380-384
Position: Home > Articles > Determination of Sudan dyes in Sichuan sausage by combination of matrix solid-phase dispersion and high performance liquid chromatography Science and Technology of Food Industry 2012,33 (9) 380-384

基质固相分散-高效液相色谱法测定川味香肠中的苏丹红染料

作  者：
杨春林;李佳峻;胡强;吴蔚;蒙辉;彭灯水
单  位：
乐山市产品质量监督检验所食品化工中心;上海海丰现代农业有限公司;四川农业大学资源环境学院
关键词：
川味香肠;苏丹红I～IV;高效液相色谱;基质固相分散
摘  要：
建立了川味香肠中苏丹红I～IV的基质固相分散(MSPD)-高效液相色谱(HPLC)分析方法。样品以无水硫酸钠作为分散基体,研磨均匀后与中性氧化铝同时装柱,正己烷淋洗净化,以丙酮-正己烷(5∶95,V/V)溶液洗脱。用InertsilODS-spC18柱(4.6mm×250mm,5μm)进行分离,流动相A为含0.1%甲酸的甲醇,流动相B为0.02mol/L的乙酸铵溶液(A∶B=85∶15,V/V),等度洗脱,柱温40℃。二极管阵列检测器检测,检测波长为490nm,利用保留时间和光谱图定性,外标法定量。4种苏丹红染料在0.10～50.00μg/mL范围内线性关系良好,相关系数均大于0.9999,苏丹红I、II、III、IV的检出限(LOD)分别为0.008～0.011mg/kg(信噪比S/N=3)。在添加浓度为5.0～25.0mg/kg范围内平均回收率达85.54%～94.66%,相对标准偏差(RSD)为0.87%～4.23%(n=6)。
译  名：
Determination of Sudan dyes in Sichuan sausage by combination of matrix solid-phase dispersion and high performance liquid chromatography
作  者：
YANG Chun-lin1,LI Jia-jun1,HU Qiang1,WU Wei1,MENG Hui2,PENG Deng-shui3(1.Center of Food and Chemical Product,Leshan Quality Supervising & Inspection Bureau,Leshan 614000,China;2.Shanghai Haifeng Modern Agricultural Co.,Ltd.,Shanghai 202179,China;3.College of Resources and Environment,Sichuan Agricultural University,Ya'an 625014,China)
关键词：
Sichuan sausage;Sudan I～IV;high-performance liquid chromatography;matrix solid-phase dispersion
摘  要：
A high-performance liquid chromatography(HPLC)method combined with matrix solid-phase dispersion(MSPD)was established for determination of Sudan I～IV in Sichuan sausage.The sample was mixed with anhydrous sodium sulfate to be a part of the chromatography column.The samples were cleaned up with hexane and rinsed with 50mL of acetone-hexane(5:95,V/V).Separation was carried out on reversed-phase Inertsil ODS-sp C18 column(4.6mm×250mm,5μm)by using a mixture of methanol and formic acid(999:1)as mobile phase A,and 0.02mol/L ammonium acetate solution as mobile phase B(A:B=85:15,V/V)at a flow rate of 1.0mL/min.Detected at the wavelength of 490nm by diode array detector as the temperature was hold at 40℃.The retention time and spectra characterization were both used to determine Sudan dyes and the content of Sudan dyes was quantified by external standard method.Sudan I～IV have good linearity in the range of 0.10～50.00μg/mL,the correlation coefficients were all above 0.9999 and the detection limits of four Sudan dyes were 0.008～0.011mg/kg(S/N=3).With the addition of 5.0 to 25.0mg/kg of Sudan standard to samples,the average recovery rates of four Sudan dyes in Sichuan sausage were between 85.54% and 94.66%.The relative standard deviations(RSDs)of spiked samples varied from 0.87% to 4.23%(n=6).

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