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当前位置: 首页 > 文章 > 耐辐射异常球菌dlp基因缺失突变株构建及其生物学功能研究 生物技术通报 2017,33 (2) 155-163
Position: Home > Articles > Construction and Biological Characterization of Gene dlp Deletion Mutant of Deinococcus radiodurans R1 Biotechnology Bulletin 2017,33 (2) 155-163

耐辐射异常球菌dlp基因缺失突变株构建及其生物学功能研究

作  者：
刘小利;江世杰;薛冬;刘盈盈;吴小丽;冯帅;韩佳慧;汪雨舟;平淑珍;王劲
单  位：
西南科技大学生命科学与工程学院;中国农业科学院生物技术研究所;中国人民大学附属中学
关键词：
耐辐射异常球菌;基因突变;亲水蛋白Dlp;非生物胁迫;酶活性
摘  要：
耐辐射异常球菌(Deinococcus radiodurans,DR)因其具有对非生物胁迫超强的抵抗能力而备受关注。旨在了解该菌亲水蛋白Dlp在细胞耐受非生物胁迫中的作用,采用融合PCR和基因同源重组技术,获得了dlp基因缺失突变株Δdlp,对野生型DR及突变株Δdlp进行非生物胁迫。结果表明,dlp的缺失导致DR细胞对高盐和氧化胁迫敏感。体外检测氧化胁迫条件下Dlp蛋白对苹果酸脱氢酶(MDH)和乳酸脱氢酶(LDH)活性的保护,结果显示,Dlp蛋白的加入能缓解氧化胁迫条件下MDH、LDH酶活性的损失。由此表明,亲水蛋白Dlp增强了耐辐射异常球菌对非生物胁迫的抗性,并能以类似分子伴侣的形式保护胁迫条件下酶的活性。
译  名：
Construction and Biological Characterization of Gene dlp Deletion Mutant of Deinococcus radiodurans R1
作  者：
LIU Xiao-li;JIANG Shi-jie;XUE Dong;LIU Ying-ying;WU Xiao-li;FENG Shuai;HAN Jia-hui;WANG Yu-zhou;PING Shu-zhen;WANG Jin;College of Life Science and Engineering,Southwest University of Science and Technology;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences;College of Life Sciences,Sichuan University;The High School Affiliated to Renmin University of China;
关键词：
Deinococcus radiodurans;;gene mutant;;hydrophilic protein Dlp;;abiotic stress;;enzyme activity
摘  要：
Deinococcus radiodurans(DR)has drawn growing attentions for its superior resistance to abiotic stress. To investigate theeffect of the hydrophilic protein Dlp in cell tolerance to abiotic stress,the dlp deletion mutant strain(Δdlp)was obtained by fusion PCR andhomologous recombination in vivo. The assays of wild strain DR and mutant Δdlp were analyzed under abiotic stress. The results showed thatthe deletion of dlp gene in D. radiodurans led cells to be more sensitive to high-salt and oxidative stresses. The in vitro protective effect of Dlpprotein on the activities of MDH and LDH was measured under oxidative stress condition;and the data demonstrated that the addition of Dlpprotein alleviated the loss of activities of MDH and LDH under oxidative stress. Our findings indicated that hydrophilic protein Dlp enhanced theresistance of D. radiodurans to abiotic stresses,and functioned as chaperone-like protein to protect enzyme activity under abiotic stresses.

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