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Position: Home > Articles > Simple Methods for Separating Lysozyme from Egg White Animal Husbandry and Feed Science 2010,31 (5) 97-99

鸡蛋清溶菌酶的简易制备方法研究

作  者:
Zhengqi W
单  位:
College of Bioengineering, Hubei University of Technology, Wuhan 430068, Chin
关键词:
lysozyme;egg white;developed;min;ammonium sulfate;metho
摘  要:
建立了一种简单有效的提纯鸡蛋清溶菌酶的方法,制备出的鸡蛋清溶菌酶纯度较高。具体方法为:用pH值为6.5的磷酸盐缓冲液(PBS)稀释蛋清5倍,搅拌30min,然后调pH值至4.6,加热至100℃并保温20min,冷却后离心,上清液进行硫酸铵分级沉淀,取40%~80%饱和度部分的沉淀,即为溶菌酶,其纯度达到电泳纯。
译  名:
Simple Methods for Separating Lysozyme from Egg White
作  者:
KANG Xu,DENG Chuan,YUAN Jiang-lan,WU Zheng-qi (College of Bioengineering,Hubei University of Technology,Wuhan 430068,China)
关键词:
egg white;lysozyme;preparation
摘  要:
A simple and efficient method was developed to separate lysozyme from egg white.And high purity lysozyme was prepared by the developed method.The steps of this method are as follows: diluting egg white with five times volume of phosphate buffer solution (PBS;pH 6.5);stirring for 30 min;adjusting pH value to 4.6;heating at 100 ° C for 20 min;cooling and centrifugation;treating supernatant by fractional precipitation with ammonium sulfate.The precipitate produced with 40%-80% saturated ammonium sulfate was electrophoresis-pure lysozyme.

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