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Position: Home > Articles > Application of quadratic regressive orthogonal rotational combinational design for regeneration study of pear cotyledon adventitious shoots Journal of Fruit Science 2011,28 (1) 171-175

二次回归正交旋转组合设计在梨子叶不定梢再生研究中的应用

作  者:
鲁敏;汤浩茹;余世杰;雷美艳;陈小杨;丁继军
单  位:
四川农业大学园艺学院
关键词:
梨;子叶;二次回归正交旋转组合设计;再生体系;数学模型
摘  要:
以水晶×金二十世纪花后90 d的梨成熟胚子叶为外植体,利用二次回归正交旋转组合设计对其再生体系研究中的关键因素6-BA、NAA、AgNO3浓度建立数学模型,得到回归方程Y=4.405 3+0.863 8X1-0.386 9X2-0.058 4X3-0.015 7X1X2+0.000 5X1X3+0.011 2X2X3-0.671 8X12-0.219 2X22-0.749 9X32。6-BA、NAA、AgNO3浓度在试验设定范围内对梨成熟胚子叶不定梢再生系数的影响均呈"低促高抑"现象;且培养基中6-BA、NAA、AgNO3的浓度分别在5.955~7.124 mg·L-1、0.281~0.473 mg·L-1和2.199~2.801 mg·L-1,梨成熟胚子叶不定梢再生系数大于3的可靠性为95%。
译  名:
Application of quadratic regressive orthogonal rotational combinational design for regeneration study of pear cotyledon adventitious shoots
作  者:
LU Min,TANG Hao-ru*,YU Shi-jie,LEI Mei-yan,CHEN Xiao-yang,DING Ji-jun(College of Horticulture,Sichuan Agricultural University,Ya'an,Sichuang 625014 China)
关键词:
Pear;Cotyledon;Quadratic regressive orthogonal rotational combinational design;Regeneration system;Mathematical model
摘  要:
Cotyledons of seeds from the cross Sujeong×Gold-Nijisseiki were taken as explants on 90 d after pollination.Mathematical model was constructed with 6-BA,NAA and AgNO3 using quadratic regressive orthogonal rotational combinational design,Y=4.405 3+0.863 8X1-0.386 9X2-0.058 4X3-0.015 7 X1X2+0.000 5X1X3+0.011 2X2X3-0.671 8X12-0.219 2X22-0.749 9X32.The results showed that the regeneration coefficient was higher when the concentration of 6-BA,NAA and AgNO3 were lower;and that the coefficient was more than 3 with the probability of 95% when 6-BA concentration was in a range of 5.955 to 7.124 mg·L-1,NAA in 0.281 to 0.473 mg·L-1 and AgNO3 in 2.199 to 2.801 mg·L-1.

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