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Position: Home > Articles > Application of Cre/loxp site-specific recombination technology on transgenic plants Fujian Journal of Agricultural Sciences 2008,23 (2) 211-217

Cre/loxp位点特异性重组系统在转基因植物中的应用

作  者:
邱淑萍;陈在杰;王锋
单  位:
福建省农业科学院福建农业遗传工程重点实验室
关键词:
位点特异性重组;Cre/loxp;转基因植物;基因删除;定点整合
摘  要:
位点特异性重组系统能够在植物遗传转化中更准确、可靠的操纵外源DNA的引入或删除,已成为植物遗传操作中的重要工具。本文简要介绍目前应用最为广泛的Cre/loxp位点特异性重组系统的重组机制,并着重阐述Cre/loxp系统在删除转基因植物中标记基因及定点整合等方面的应用。
译  名:
Application of Cre/loxp site-specific recombination technology on transgenic plants
作  者:
QIU Shu-ping1,2,CHEN Zai-jie1,WANG Feng1(1.Fujian Key Laboratory of Genetic Engineering for Agriculture,Fujian Academy of Agricul-tural Sciences,Fuzhou,Fujian 350003,China;2.College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350007,China)
关键词:
site-specific recombination;Cre/loxp;transgenic plant;gene deletion;site-specific integration
摘  要:
Site-specific recombination technology has been developed and widely applied for plant genetic manipulations.It can successfully delete marker genes from a transgenic plant or site-specifically integrate a gene to a pre-determined genomic location in a precise,single-copy pattern.This article reviews the Cre/loxp site-specific recombination technique and its applications for marker gene deletion and precise gene integration.

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