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当前位置: 首页 > 文章 > 测定昆虫卵巢内核酸、蛋白质等含量的方法研究 山东农业大学学报(自然科学版) 1987 (4) 49-54
Position: Home > Articles > STUDY ON THE DETERMING METHOD OF NUCLEIC ACID, PROTEIN AND SO ON IN INSECT OVARIES Journal of Shandong Agricultural University(Natural Science Edition) 1987 (4) 49-54

测定昆虫卵巢内核酸、蛋白质等含量的方法研究

作  者：
张韵梅
单  位：
山东农业大学基础部
关键词：
昆虫;生物样品分析;分离;DNA;RNA;蛋白质
摘  要：
本文在研究昆虫雌虫卵巢内无机磷、游离核苷酸、磷脂、RNA、DNA、磷蛋白和蛋白质的含量时,将Schnider分离法、Schmidt和Thannhauser分离法结合,并进行改进。在用NaOH降解RNA后,改用HCl代替PCA或TCA,使之生成的NaCl(浓度为0.15N),在此溶液中DNA溶解度很小,更利于分离RNA与DNA。并以定磷法和紫外吸收法对比测定RNA含量,以Folin-phenal法与紫外吸收法对比测定蛋白质含量,表明改进的分离方法测定的结果准确,方法简便,取材少,可一次性分离上述7种成份。
译  名：
STUDY ON THE DETERMING METHOD OF NUCLEIC ACID, PROTEIN AND SO ON IN INSECT OVARIES
作  者：
Zhang Yunmei (The Department of Basic Courses, Shandong Agricultural University)
关键词：
insect;;biological sample analysis;;separate, DNA, RNA, protein.
摘  要：
Studying contents of inorganic phosphorus, free nucleotide, phospholipid, DNA, RNA, phosphoprotein and protein in ovaries of Haplotropis brunnerian Saus. and Liacala brevitarsis Lewis, the author connected the Schnider separating method with the Schmidt & Thannhauser methods thus improved the determing method. After degradation of RNA with NaOH, the HCl was used in replacement of PCA and TCA to force the NaCl produced (cone. 0.15 N). In this solution, the solubility of the DNA was negligible, so more favourable to separate RNA and DNA. The contents of RNA determined by the modified method were compared with that by the phospho-fixing method and ultraviolet-absorption method, and the protein contents by Folin-phenal method and ultraviolet-absorption method. The results showed that the modified separating method is accurate, simple, less materials required and could separate seven components at the same time.

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