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当前位置: 首页 > 文章 > 环介导等温扩增技术检测食品中酸土环脂芽孢杆菌 食品科学 2014,35 (22) 233-237
Position: Home > Articles > Loop-Mediated Isothermal Amplification(LAMP) for Detection of Alicyclobacillus acidoterrestris in Foods FOOD SCIENCE 2014,35 (22) 233-237

环介导等温扩增技术检测食品中酸土环脂芽孢杆菌

作  者：
周赞虎;张海艳;郑俊超;江喆敏;陈春香;何艺贞;林俊君;苏雪勤;王根芳
单  位：
厦门大学生命科学学院;漳州出入境检验检疫局;漳州卫生职业学院
关键词：
环介导等温扩增;酸土环脂芽孢杆菌;食品
摘  要：
目的:利用环介导等温扩增技术建立食品中酸土环脂芽孢杆菌快速检测方法。方法:针对酸土环脂芽孢杆菌16S序列设计特异引物,再优选反应体系,用显色法检测实验结果。结果:该方法能够在63℃条件下1 h内检出食品中酸土环脂芽孢杆菌,所设计的引物有良好的特异性;灵敏度达6.7 CFU/m L(弱阳性)。结论:该方法具有高效、特异性强和敏感性高等特点,可满足酸土环脂芽孢杆菌快速检测筛选的要求。
译  名：
Loop-Mediated Isothermal Amplification(LAMP) for Detection of Alicyclobacillus acidoterrestris in Foods
作  者：
ZHOU Zan-hu;ZHANG Hai-yan;ZHENG Jun-chao;JIANG Zhe-min;CHEN Chun-xiang;HE Yi-zhen;LIN Jun-jun;SU Xue-qin;WANG Gen-fang;ZHENG Tian-ling;Key Laboratory of Coastal and Wetland Ecosystems, Ministry of Education, School of Life Sciences, Xiamen University;Zhangzhou Entry-Exit Inspection and Quarantine Bureau;College of Zhangzhou Health Vocational;
关键词：
loop-mediated isothermal amplification(LAMP);;Alicyclobacillus acidoterrestris;;food
摘  要：
Purpose: A loop-mediated isothermal amplification(LAMP) method was established for the detection of Alicyclobacillus acidoterrestris in foods. Methods: After optimization of the reaction conditions of LAMP including the concentrations of primers, reaction time and amplification temperature, the LAMP method was developed, and its sensitivity and specificity were evaluated. Results: The method was capable of rapidly and specifically detecting A. acidoterrestris in foods within 1 hour at a constant temperature of 63 ℃. The sensitivity of the method was 6.7 CFU/m L and the specificity was 100%. Conclusions: The LAMP method is efficient, highly sensitive and specific, and suitable for the rapid detection of A. acidoterrestris in various food samples.

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