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当前位置: 首页 > 文章 > 钝裂银莲花花粉活力测定方法的研究 植物研究 2013,33 (5) 72-76
Position: Home > Articles > Detection Methods for Pollen Viability of Anemone obtusiloba Bulletin of Botanical Research 2013,33 (5) 72-76

钝裂银莲花花粉活力测定方法的研究

作  者：
胡春;刘左军;李富香;刘凤梅
单  位：
兰州理工大学生命科学与工程学院
关键词：
钝裂银莲花;测定方法;可育性;花粉;活力
摘  要：
以3种花色(黄色、浅黄色和白色)钝裂银莲花为研究对象,分别采用TTC染色、I2-KI染色、无机酸测定和蔗糖萌发4种方法,测定了钝裂银莲花(Anemone obtusiloba)的花粉活力,同时比较了不种方法对花粉活力的测定效果,并了解了花粉活力在一天内的动态变化情况。结果表明:(1)钝裂银莲花花粉可育率很高,达80%左右。花粉直径为28.75~33.75μm。(2)TTC浓度影响花粉活力的测定,当浓度为0.5%时,所测花粉活力的效果较好。(3)I2-KI法的染色效果不理想,染色率仅为8.33%~22.2%,且3种花色花粉活力测定数据间差异不显著。(4)3种花色钝裂银莲花花粉活力有差异,黄色最高,浅黄色次之,白色最低。TTC染色法是简单快速测定钝裂银莲花花粉活力的最适方法。一天内花粉活力在12:00~14:00最大。
译  名：
Detection Methods for Pollen Viability of Anemone obtusiloba
作  者：
HU Chun;LIU Zuo-Jun;LI Fu-Xiang;LIU Feng-Mei;School of Life Science and Engineering of Lanzhou University of Technology;
关键词：
Anemone obtusiloba;;detection methods;;fertility;;pollen;;viability
摘  要：
The pollen viability of Anemone obtusiloba with three-color flowers( yellow,pale yellow and white) was detected with four methods: TTC dyeing,I 2-KI dyeing,inorganic acid test,sucrose-based germination,the determination effects of different methods were compared,and the dynamic change of pollen viability within a day was understood. The results show that:( 1) The fertile rate of pollen of A. obtusiloba is high,about 80%. Pollens diameter are 28. 75- 33. 75 μm.( 2) The concentration of TTC can influence the determination of pollen vitality,when the concentration was 0. 5%,it was better than the others.( 3) I 2-KI dyeing is not ideal,dyeing rate is only 8. 33%- 22. 2%,and there is no significant difference within three-color flowers.( 4) The pollen vitality of A. obtusiloba with three-color flowers have differences,with yellow the highest,followed by light yellow,white the minimum. TTC dyeing is a simple and rapid determination method of A. obtusiloba. Within a day,pollen vitality is maximum from 12: 00- 14: 00 pm.

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