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聚乙二醇修饰对牛乳β-乳球蛋白抗原性的影响

作  者:
蔡小飞;钟俊桢;刘成梅;刘伟;徐雨佳;罗舜菁
单  位:
南昌大学食品科学与技术国家重点实验室
关键词:
β-乳球蛋白;聚乙二醇;ELISA;抗原性
摘  要:
通过不同修饰反应物质的量比、修饰反应时间、pH值,用单甲氧基聚乙二醇琥珀酰亚胺酯(SC-mPEG)对牛乳β-乳球蛋白(β-LG)进行修饰,研究其抗原性的变化。利用间接竞争ELISA检测结合产物的抗原性,结果显示不同条件下PEG修饰后的β-乳球蛋白的抗原性最高降低率,反应8h时为70.2%。三硝基苯磺酸(TNBS)法测定不同条件反应后样品修饰度,反应8h时最高修饰度为39.1%。结果表明修饰反应时间为SC-mPEG修饰β-LG后其抗原性降低的主要因素。
译  名:
Effect of PEG Modification on Antigenicity of β-Lactoglobulin
作  者:
CAI Xiao-fei,ZHONG Jun-zhen,LIU Cheng-mei,LIU Wei,XU Yu-jia,LUO Shun-jing (State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China)
关键词:
β-lactoglobulin;polyethylene glycol;ELISA;antigenicity
摘  要:
β-Lactoglobulin(β-LG) was modified by suecinimidyl carbonate methoxy polyethlene glycol(SC-mPEG) under different conditions of molar ratio,reaction time and pH.The change of its antigenicity was also studied.Indirect competitive ELISA was used to detect the antigenicity of the SC-mPEG-β-LG mixture.The results indicated that the highest reduction rate of antigenicity for modified products under different conditions was 70.2% at the reaction time of 8 h and at the same time,the highest modification degree detected by TNBS was 39.1%.Meanwhile,reaction time was the major factor affecting antigenicity reduction of β-LG modified by SC-mPEG.

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