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Position: Home > Articles > Affinity Purification of Rabbit IgG Against Buffalo's β-lactoglobulin FOOD SCIENCE 2007,28 (8) 276-279

抗水牛乳β-乳球蛋白兔IgG的亲和纯化

作  者:
李欣;陈红兵
单  位:
南昌大学食品科学教育部重点实验室
关键词:
β-乳球蛋白;IgG;亲和层析
摘  要:
为得到兔血清中纯度较高的特异性抗体,通过将纯化的水牛乳β-乳球蛋白耦联到免疫亲和柱上,用3mol/L氯化镁将特异性的IgG洗脱,得到了SDS-PAGE纯的IgG,其蛋白含量为5.8mg/ml,经ELISA检测滴度为1:109。本实验所建立的方法可用于纯化兔血清中特异性的IgG。
译  名:
Affinity Purification of Rabbit IgG Against Buffalo's β-lactoglobulin
作  者:
LI Xin1,2,CHEN Hong-bing1,2, (1.Key Laboratory of Food Science, Ministry of Education,Nanchang University, Nanchang 330047, China; 2.Sino-German Joint Research Institute, Nanchang University, Nachang 330047, China)
关键词:
β-lactoglobulin;IgG;affinity chromatography
摘  要:
In order to collect high purity rabbit antibody against buffola's β-lactoglobulin, β-lactoglobulin was coupled into the medium of Sepharose 4B, and then the medium was eluted with 3 mol/L MgCl2. The results showed that electrophoretically pure of IgG is identified by SDS-PAGE, the protein content is 5.8 mg/ml, and the titer is 1:109 using ELISA. So the method established can be used to purify the specific IgG in rabbit serum.

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