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Position: Home > Articles > Study on Rapid Detection of Food-borne Pathogens in Meat by Multiplex PCR and Its Kit FOOD SCIENCE 2008,29 (6) 255-258

肉品中致病菌的多重PCR检测及固相化试剂盒的研究

作者：

陈伟;李正国;杨平;杨迎伍;邓伟;王国民

单位：

重庆大学生物工程学院基因工程研究中心

关键词：

肉制品;食源性致病菌;多重PCR;试剂盒

摘要：

沙门氏菌、志贺氏菌和大肠杆菌O157:H7是肉品中的重要食源性致病菌,建立其快速检测方法对肉品的质量控制具有重要作用。本研究根据沙门氏菌的invA基因、志贺氏菌的ipaH基因以及大肠杆菌O157:H7的rfbE基因,分别设计出三对特异性引物,通过对多重PCR反应体系和条件的优化,建立多重PCR检测体系,并集成快速检测试剂盒。结果表明,该方法简单快速且灵敏度高,在肉品中的检测灵敏度可达1CFU/g,整个检测时间在24h以内。集成的试剂盒检测性能良好,具有较强应用价值,可推广应用于食品卫生检测以及临床检验等领域。

译名：

Study on Rapid Detection of Food-borne Pathogens in Meat by Multiplex PCR and Its Kit

作者：

CHEN Wei,LI Zheng-guo,YANG Ping,YANG Ying-wu,DENG Wei,WANG Guo-min (Genetic Engineering Research Center, College of Bio-Engineering, Chongqing University, Key Laboratory of Functional Gene and New Regulation Technologies under Chongqing Municipal Education Commission, Chongqing 400044, China)

关键词：

meat product;food-borne pathogenic microorganism;multiplex PCR;detection kit

摘要：

Salmonella, Shigella and Escherichia coli O157:H7 are important food-borne pathogens in meat and its products, therefore it is very useful to establish rapid detection method for controlling the quality of meat and its products. Three sets of specific primers were designed according to the gene segment of invA of Salmonella, ipaH of Shigella and rfbE of Escherichia coli O157:H7, and then the reaction parameters were optimized to develop a PCR detection kit. The results showed that the multiplex PCR method is simple and rapid, the sensitivity achieved is as low as 1 CFU/g and the whole detection time is less than 24 hours. The capacity of the detection kit is rapid and accurate, so it could be wildly used in many fields such as food sanitation detection, clinical inspection and so on.

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肉品中致病菌的多重PCR检测及固相化试剂盒的研究

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