中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 非离子表面活性剂萃取-高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚 食品科学 2012,33 (6) 150-153
Position: Home > Articles > Simultaneous Determination of Chlorogenic Acid,Quercetin and Kaempferol in Loquat Leaves by Non-ionic Surfactant Extraction Solvent Followed by High Performance Liquid Chromatography FOOD SCIENCE 2012,33 (6) 150-153

非离子表面活性剂萃取-高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚

作  者：
张丽贤;周光明;姜兰芳;许丽
单  位：
发光与实时分析教育部重点实验室西南大学化学化工学院
关键词：
枇杷叶;Triton X-100表面活性剂;高效液相色谱(HPLC);绿原酸;槲皮素;山奈酚
摘  要：
目的:以5g/100mL非离子表面活性剂Triton X-100为萃取剂,建立一种简单快速的采用超声提取,高效液相色谱法测定枇杷叶中的绿原酸、槲皮素和山奈酚的方法。方法:采用Phenomenex C18色谱柱(250mm×4.6mm,5.0μm),以甲醇-0.25%乙酸溶液为流动相梯度洗脱,流速1.0mL/min,紫外检测波长350nm。结果:绿原酸、槲皮素和山奈酚分别在0.011～220μg/mL(r=0.9997)、0.065～130μg/mL(r=0.9999)、0.068～136μg/mL(r=0.9999)线性关系良好,检出限(RSN=3)依次是1.94、0.22、0.27ng/mL;样品的加标回收率为90.40%～96.82%。结论:该方法具有样品前处理简单、绿色环保、灵敏快速等优点,可为枇杷叶中一些黄酮类物质和绿原酸的检测提供一个有效的方法。
译  名：
Simultaneous Determination of Chlorogenic Acid,Quercetin and Kaempferol in Loquat Leaves by Non-ionic Surfactant Extraction Solvent Followed by High Performance Liquid Chromatography
作  者：
ZHANG Li-xian,ZHOU Guang-ming,JIANG Lan-fang,XU Li(Key Laboratory on Luminescence and Real-Time Analysis,Ministry of Education,School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)
关键词：
folium eriobotryae;Triton X-100 surfactant;high performance liquid chromatography(HPLC);chlorogenic acid;quercetin;kaempferol
摘  要：
Objective:An HPLC method for the simultaneous determination of chlorogenic acid,quercetin and kaempferol in loquat leaves was proposed based on rapid ultrasonic-assisted extraction using the non-ionic surfactant Triton X-100 as the extractant.Methods:The chromatographic separation was achieved on a Phenomenex C18 column using a mobile phase made up of methanol and 0.25% acetic acid at a flow rate of 1.0 mL/min,and the analytes were detected with a UV detector at 350 nm.Results:The calibration curves for chlorogenic acid,quercetin and kaempferol were linear in the range of 0.011-220μg/mL(r=0.9997),0.065-130μg/mL(r=0.9999),0.068-136μg/mL(r=0.9999),respectively.The limit of detection(LOD) was 1.94,0.22,0.27 ng/mL(RSN=3),respectively.The average recoveries across three spike levels ranged from 90.71% to 94.88%(n=3).Conclusion:The method is simple,rapid,convenient,sensitive,and environment-friendly,and thus can provide a new and scientific means for assaying the contents of flavonoids and chlorogenic acid in loquat leaves.

相似文章

导出引用
计量
文章访问数: 18
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46