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Position: Home > Articles > Isolation and identification of marine Vibrio from Xisha Yongle Islands, China Journal of Fisheries Research 2022,44 (1) 1-8

西沙永乐群岛海域弧菌的分离鉴定

作  者：
张秀霞;张泽龙;李军涛;鲁耀鹏;郑佩华;冼健安
单  位：
中国热带农业科学院热带生物技术研究所海南省海洋生物资源功能性成分研究与利用重点实验室;中国热带农业科学院海南热带农业资源研究院
关键词：
弧菌;鉴定;16S rDNA;HSP60;gyrB
摘  要：
从西沙永乐群岛7个岛礁海域的海水中分离出47株弧菌,将47株菌株进行16S rDNA、HSP60和gyrB扩增,序列在Genebank中进行比对,构建系统发育树。结果显示利用16S rDNA和HSP60序列比对均无法鉴定弧菌到种。gyrB1和gyrB2引物均只能扩增出6株菌株的目标序列,gyrB3引物能扩增47株菌株的目标序列,且成功构建系统发育树,可鉴定弧菌到种。47株弧菌菌株分属于7个种,鸭公岛弧菌多样性最丰富,可分离到5种;石屿和羚羊礁筐仔沙洲弧菌多样性最低,均只分离到2种。本研究建立了可靠的海洋弧菌的分子鉴定方法,获得的菌株丰富了南海微生物菌种库,可为今后的研究与教学提供材料。
译  名：
Isolation and identification of marine Vibrio from Xisha Yongle Islands, China
作  者：
ZHANG Xiuxia;ZHANG Zelong;LI Juntao;LU Yaopeng;ZHENG Peihua;XIAN Jian'an;Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Bioscience and Biotechnology,CATAS;Hainan Institute of Tropical Agricultural Resources,CATAS;
关键词：
Vibrio;;identification;;16S rDNA;;HSP60;;gyrB
摘  要：
Forty-seven strains of Vibrio were isolated from sea area of 7 islands of Xisha Yongle Islands.The 16 S rDNA,HSP60 and gyrB genes of the 47 strains were amplified, and their sequences were compared in GeneBank and then phylogenetic trees were constructed.The results showed that neither 16 S rDNA nor HSP60 could be used to identify the species of Vibrio.Both gyrB1 and gyrB2 primers could only amplify the target sequence of 6 strains.gyrB3 primers could amplify the target sequence of all the 47 strains, and the phylogenetic trees were successfully constructed.Species could be identified using gyrB3 primers.The 47 strains of Vibrio were divided into 7 species.Five strains of Vibrio was isolated in Yagong Island which had the highest diversity.Two species were isolated in both Shiyu and Antelope reef which had the lowest diversity.In this study, a reliable molecular identification method for marine Vibrio was established.The obtained strains enriched the microbial library of the South China Sea and provided materials for future research and teaching.

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