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食品中五种致病性弧菌MPCR-DHPLC检测方法的建立

作  者:
邵碧英;王传得;郑晶;黄晓蓉;曹际娟;陈彬;吴谦
单  位:
福建出入境检验检疫局;福建农林大学动物科学院;辽宁出入境枪验检疫局
关键词:
弧菌;基因组DNA;多重PCR;变性高效液相色谱
摘  要:
采用试剂盒法提取5种致病性弧菌(霍乱弧菌、副溶血性弧菌、创伤弧菌、溶藻弧菌和拟态弧菌)的基因组DNA。分别合成5对特异性引物,对PCR反应体系进行优化后,建立了5种致病性弧菌间的MPCR-DHPLC检测方法。二重PCR-DHPLC检测灵敏度可达到100CFU/ml。建立的MPCR-DHPLC方法在食品中弧菌的检测上具有很好的应用价值。
译  名:
Development of MPCR-DHPLC Detection Method for Five Pathogenic Vibrios in Foods
作  者:
SHAO Bi-ying1,WANG Chuan-de2,ZHENG Jing1,HUANG Xiao-rong1,Cao Ji-juan3,CHEN Bin1,WU Qian1 (1.Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou 350001,China; 2.College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China; 3.Liaoning Entry-Exit Inspection and Quarantine Bureau,Dalian 116001,China)
关键词:
Vibrio;genome DNA;multiplex PCR (MPCR);denaturalization high performance liquid chromatography (DHPLC)
摘  要:
The genome DNAs of five pathogenic Vibrios were extracted by kit method,including Vibrio cholerae,V. parahaemolyticus,V. vulnificus,V. alginolyticus and V. mimicus,and their five pairs of special primers were composed,respectively. The MPCR-DHPLC detection methods among the five vibrios were developed after the PCR reaction systems were optimized. The detection sensitivity of duplex PCR-DHPLC is 100 CFU/ml. The developed MPCR-DHPLC method has good application merit on detecting the Vibrios in foods.

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