中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 白鲢鱼肌原纤维蛋白双向电泳分析体系的建立 食品科学 2017,38 (1) 41-46
Position: Home > Articles > Establishment of Two-Dimensional Gel Electrophoresis System for Myofibrillar Proteins of Silver Carp(Hypophthalmichthys molitrix) FOOD SCIENCE 2017,38 (1) 41-46

白鲢鱼肌原纤维蛋白双向电泳分析体系的建立

作  者：
仪淑敏;李睿智;陈杨;励建荣;李学鹏;徐永霞;李春;丁浩宸;郁晓君
单  位：
渤海大学食品科学与工程学院;渤海大学数理学院;辽宁安井食品有限公司
关键词：
白鲢鱼;肌原纤维蛋白;双向电泳
摘  要：
以白鲢鱼为研究对象,建立并优化其肌原纤维蛋白的双向电泳技术。结果表明,选用固相p H值梯度预制胶条p H 4~7,上样量120μg,等电聚焦程序Ⅲ(50 V主动水化15 h,250 V、2 h,1 000 V、2.5 h及4 000 V、3 h三段式除盐,8 000 V、2 h和10 000 V、1.5 h两段式升压,10 000 V聚焦80 000 V·h,500 V保持10 h)以及12%的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离胶条件下进行双向电泳,凝胶银染后用PDQuest软件分析,得到具有高分离度的肌原纤维蛋白双向电泳图谱,蛋白点清晰。
译  名：
Establishment of Two-Dimensional Gel Electrophoresis System for Myofibrillar Proteins of Silver Carp(Hypophthalmichthys molitrix)
作  者：
YI Shumin;LI Ruizhi;CHEN Yang;LI Jianrong;LI Xuepeng;XU Yongxia;LI Chun;DING Haochen;YU Xiaojun;College of Food Science and Engineering, Bohai University;College of Mathematics and Physics, Bohai University;Liaoning Anjoy Food Co.Ltd.;
关键词：
silver carp;;myofibrillar protein;;two-dimensional gel electrophoresis(2-DE)
摘  要：
A two-dimensional gel electrophoresis(2-DE) system for the separation of myofibrillar proteins from silver carp was established and optimized in this study. The key factors of isoelectric focusing(IEF) and the p H gradient of immobilized p H gradient(IPG) strip were selected as the main factors affecting 2-DE in this paper. We loaded 120 μg of protein onto the 17 cm p H 4-7 IPG strip, and the IEF program III was as follows: 50 V for 15 h, three-stage desalting involving 250 V for 2 h, 1 000 V for 2.5 h and 4 000 V for 3 h, 8 000 V for 2 h and 10 000 V for 1.5 h, and 10 000 V for 80 000 V·h and 500 V held for 10 h. The gel concentration was 12%. The gel was stained with sliver nitrate, scanned and analyzed using PDQuest software. A high-resolution 2-DE map for myofibrillar proteins from silver carp was obtained finally.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46