译 名：
Extraction and Determination of Peptaibol a and b from Mycelia of Trichoderma citrinoviride
作 者：
CHEN Juan;SONG Bang-le;KUANG Wen-feng;MAO Wei-li;Shanghai WLH Bio-Tech Company;Shanghai Jiaotong University School of Agriculture and Biology;
单 位：
Shanghai WLH Bio-Tech Company%Shanghai Jiaotong University School of Agriculture and Biology
关键词：
Trichoderma citrinoviride;;extraction;;detection;;secondary metabolite
摘 要：
[Aims] The aims were to establish a methods for extraction and determination of the secondary metabolites of peptaibol a and b from mycelia of Trichoderma citrinoviride during the process of liquid fermentation.[Methods] The single factor and orthogonal test were conducted to screen and optimize the impact factors such as concentration of ethanol,ratio of liquid to solid,extraction time and temperature.The detection of peptaibol a and b was conducted by HPLC used Venusil MP C_(18) column with column temperature of 40 ℃ at 205 nm,in addition,the mixture of acetonitrile and water(60∶40,by vol) was used as the mobile phase with a flow rate of 1.0 m L/min.[Results] The optimized parameters for extraction were: 70% of ethanol,ratio of liquid to solid(v/w) 5:1,room temperature for 100 min.The linear ranges for peptaibol a and b were 10-150 mg/L and 9.1-137 mg/L,the average recoveries were 100.1%(RSD=1.6%)and 99.6%(RSD=2.0%),the contents of the crude peptaibol a and b were 232 and 210 mg/g,respectively.[Conclus ion] The method is simple and reliable,which can accurately detect the contents of peptaibol a and b generated by mycelia of T.citrinoviride.
